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dc.contributor.authorWang, Hongshengen_US
dc.date.accessioned2007-05-18T12:12:47Z
dc.date.available2007-05-18T12:12:47Z
dc.date.issued1998-05-01T00:00:00Zen_US
dc.identifier.urihttp://hdl.handle.net/1993/1574
dc.description.abstractExtensive evidence supports a role for polyclonal serum natural antibody (NAb) acting as a mediator of natural resistance against tumors in mice. However, little is known about its mechanisms of action or about the phenotype of susceptible cells. C3H 10T${1\over 2}$ fibroblasts overexpressing an activated ras oncogene or a PKC-$\beta$1 gene increased their NAb binding capacities, identifying PKC, an integral signaling molecule of normal cellular activation, as a key regulator of NAb binding structures. This, coupled with corresponding decreases in expression of membrane PKC-$\alpha$ and NAb binding in resting confluent 10T${1\over 2}$ cells raised the possibility that, in general, cells activated through PKC are NAb sensitive. In addition, NAb interaction with 10T${1\over 2}$ variants initiated a signal transduction mechanism including activation of PKC, shedding of cell surface molecules and bound NAb, a reduction in phosphotyrosine levels of a membrane-associated 60 KDa molecule, and, over time, the inhibition of DNA synthesis. Together with the increased in vivo elimination of the high NAb binding PKC-$\beta$1-overexpressing cells and the beneficial effect of passive syngeneic NAb in the rejection of syngeneic tumors injected s.c. and i.v. in both xid-bearing B cell deficient and B cell normal mouse models, the data argued that NAb not only participates in tumor surveillance of preneoplasia and neoplasia but contributes to homeostasis of the organism.en_US
dc.format.extent13892530 bytes
dc.format.extent184 bytes
dc.format.mimetypeapplication/pdf
dc.format.mimetypetext/plain
dc.language.isoengen_US
dc.rightsinfo:eu-repo/semantics/openAccess
dc.titleNatural antibody recognition, signaling and surveillance in v-Ha-ras- and PKC-B1-overexpressing 10T1/2 fibroblastsen_US
dc.typeinfo:eu-repo/semantics/doctoralThesis
dc.typedoctoral thesisen_US
dc.degree.disciplineImmunologyen_US
dc.degree.levelDoctor of Philosophy (Ph.D.)en_US


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