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dc.contributor.supervisor Jackson, Alan (Medical Microbiology) en_US
dc.contributor.author Alandijany, Thamir Abdulaziz A
dc.date.accessioned 2013-01-07T14:02:31Z
dc.date.available 2013-01-07T14:02:31Z
dc.date.issued 2013-01-07
dc.identifier.uri http://hdl.handle.net/1993/14406
dc.description.abstract Infection with challenge virus standard-11 (CVS) strain, a laboratory fixed rabies virus strain, induces neuronal process degeneration in both in vivo and in vitro models. CVS-induced axonal swellings of primary rodent dorsal root ganglion neurons are associated with 4-hydroxy-2-nonenal staining indicating a critical role of oxidative stress. Mitochondrial dysfunction is one of the most important causes of oxidative stress. We hypothesized that CVS infection induces mitochondrial dysfunction leading to oxidative stress. We investigated the effects of CVS infection on several mitochondrial parameters in different cell types. CVS infection increased electron transport chain capacity, Complex I and IV activities, but did not affect Complex II-III, citrate synthase, and malate dehydrogenase activities. CVS maintained normal oxidative phosphorylation capacity and proton leak, indicating a tight mitochondrial coupling. Possibly as a result of enhanced Complex activity and efficient coupling, a high mitochondrial membrane potential was generated. CVS infection reduced the intracellular ATP level and altered the cellular redox state as indicated by high NADH/NAD+ ratio. CVS infection was associated with a higher rate of hydrogen peroxide production. We conclude that CVS infection induces mitochondrial dysfunction leading to ROS overgeneration, oxidative stress and neuronal process degeneration. en_US
dc.rights info:eu-repo/semantics/openAccess
dc.subject rabies en_US
dc.subject oxidative stress en_US
dc.subject rabies pathogenesis en_US
dc.subject reactive oxygen species en_US
dc.title Mitochondrial dysfunction in rabies virus infection of neurons en_US
dc.type info:eu-repo/semantics/masterThesis
dc.degree.discipline Medical Microbiology en_US
dc.contributor.examiningcommittee Coombs, Kevin (Medical Microbiology) Fernyhough, Paul (Pharmacology and Therapeutics) en_US
dc.degree.level Master of Science (M.Sc.) en_US
dc.description.note February 2013 en_US


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