Home

Sarcoplasmic reticulum ATPase and sarcolemmal calcium(2+)-ATPase messenger RNA expression during in vitro skeletal muscle cell differentiation

Show simple item record

dc.contributor.author Zhang, Jing. en_US
dc.date.accessioned 2007-05-17T12:34:29Z
dc.date.available 2007-05-17T12:34:29Z
dc.date.issued 1997-11-01T00:00:00Z en_US
dc.identifier.uri http://hdl.handle.net/1993/1381
dc.description.abstract Three genes coding sarcoplasmic reticulum Ca$\sp{2+}$-ATPases (SERCA) and at least four genes coding sarcolemmal Ca$\sp{2+}$-ATPase (PMCA) have been isolated and characterized. The objective of this work was to study the mRNA expression for Ca$\sp{2+}$-ATPase isoforms during in vitro differentiation of skeletal muscle cell lines. To analyze the mRNA expression pattern of the SERCA and PMCA isoforms, three skeletal muscle cell lines (L6, C2C12, Sol8) were used as models. The mRNAs of these Ca$\sp{2+}$-ATPase gene were detected by a semi-quantitatively RT-PCR technique. It is generally regarded that myogenic factors such as MyoD and myogenin can irectly regulate muscle-specific gene expression both in vivo and in vitro. In order to understand the relationship between the expression of MyoD and myogenin their mRNA levels was also measured during the differentiation process. The drugs 5-azacytidine (AZA) and 5-bromodeoxyuridine (BUdR) were used to further analyze the role of the above myogenic determination factors on the expression of the SR and SL Ca$\sp{2+}$-ATPases. (Abstract shortened by UMI.) en_US
dc.format.extent 5520970 bytes
dc.format.extent 184 bytes
dc.format.mimetype application/pdf
dc.format.mimetype text/plain
dc.language en en_US
dc.language.iso en_US
dc.title Sarcoplasmic reticulum ATPase and sarcolemmal calcium(2+)-ATPase messenger RNA expression during in vitro skeletal muscle cell differentiation en_US
dc.degree.discipline Physiology en_US
dc.degree.level Master of Science (M.Sc.) en_US


Files in this item

This item appears in the following Collection(s)

Show simple item record

View Statistics