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dc.contributor.supervisor Stasolla, Claudio (Plant Science) en_US
dc.contributor.author Yang, Cunchun
dc.date.accessioned 2012-12-17T20:20:54Z
dc.date.available 2012-12-17T20:20:54Z
dc.date.issued 2012-05 en_US
dc.date.issued 2012-09 en_US
dc.identifier.citation Elhiti et al. Altered seed oil and glucosinolate levels in transgenic plants overexpressing the Brassica napus SHOOTMERISTEMLESS gene J. Exp. Bot. (2012) 63(12): 4447-4461 first published online May 4, 2012 Elhiti et al. Transcriptional changes of antioxidant responses, hormone signalling and developmental processes evoked by the Brassica napus SHOOTMERISTEMLESS during in vitro embryogenesis. Plant Physiology and Biochemistry, Volume 58, September 2012, Pages 297–311 en_US
dc.identifier.uri http://hdl.handle.net/1993/13697
dc.description.abstract The meristem genes SHOOT MERISTEMLESS (STM), CLAVATA1 (CLV1) and ZWILLE (ZLL) are essential for the formation and maintenance of the shoot apical meristem (SAM). As an extension of that work, this thesis examines the function of these genes during seed oil accumulation and microspore-derived embryogenesis. Using a transgenic approach it is demonstrated that only the over-expression of BnSTM increases the percentage of total seed oil and a reduction of glucosinolate (GLS) levels achieved by transcriptional regualtion. The over-expression of BnSTM also affected in vitro embryogenesis by increasing the number and quality of microspore-derived embryos (MDEs) in contrast to the MDEs down-regulating BnSTM. The MDEs with ectopic expression of BnSTM were found to regulate embryonic SAM by altering cytokinin synthesis, catabolism, perception and signaling. Taken together, these findings provide evidence for a novel function of BnSTM in promoting desirable changes in seed oil and GLS levels and enhancing in vitro embryogenesis. en_US
dc.rights info:eu-repo/semantics/openAccess
dc.subject meristem en_US
dc.subject Brassica napus en_US
dc.title The effects of altered expression of meristem genes in Brassica napus en_US
dc.type info:eu-repo/semantics/masterThesis
dc.degree.discipline Plant Science en_US
dc.contributor.examiningcommittee Stasolla, Claudio (Plant Science), Ayele, Belay (Plant Science) Renault, Sylvie (Biological Sciences) en_US
dc.degree.level Master of Science (M.Sc.) en_US
dc.description.note February 2013 en_US


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