Home

A collisional damping interface for a time-of-flight mass spectrometer with both electrospray ionization and matrix assisted laser desorption ionization

Show simple item record

dc.contributor.author Krutchinsky, Andrew en_US
dc.date.accessioned 2007-05-17T12:33:30Z
dc.date.available 2007-05-17T12:33:30Z
dc.date.issued 1998-07-01T00:00:00Z en_US
dc.identifier.uri http://hdl.handle.net/1993/1352
dc.description.abstract A collisional damping interface for orthogonal injection of either electrospray ionization (ESI) or matrix-assisted laser desorption/ionization (MALDI) ions into a reflecting TOF mass spectrometer (MANITOBA TOF III) has been designed. Ions produced by either ESI or MALDI are injected into an RF-quadrupole ion guide operating at pressure $\sim$70mTorr. As they pass through the quadrupole their motion is constrained by the RF field. Meanwhile, they lose energy by collisions with the buffer gas molecules. Computer simulations and experiments performed in the instrument with the new interface indicate that collisional cooling of ions improves the quality of the primary ion beams orthogonally injected into the mass spectrometer. Important characteristics of the mass spectrometer, such as resolution and sensitivity were greatly improved. In addition, mass discrimination was reduced, providing an observable mass range greater than 1 MDa with an electrospray ion source and up to $\sim$12,300 Da with a MALDI ion source. These improvements appeared to be very useful for the study of large biological compounds, noncovalent complexes in particular. The results of investigation of several noncovalent complexes are presented to illustrate the capabilities of the instrument with the new interface. The system studied consist of Echerichia coli citrate synthase dimers and hexamers interacting with an allosteric inhibitor, the reduced form of nicotinamide adenine nucleotide. en_US
dc.format.extent 7646924 bytes
dc.format.extent 184 bytes
dc.format.mimetype application/pdf
dc.format.mimetype text/plain
dc.language en en_US
dc.language.iso en_US
dc.title A collisional damping interface for a time-of-flight mass spectrometer with both electrospray ionization and matrix assisted laser desorption ionization en_US
dc.degree.discipline Physics & Astronomy en_US
dc.degree.level Doctor of Philosophy (Ph.D.) en_US


Files in this item

This item appears in the following Collection(s)

Show simple item record

View Statistics