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dc.contributor.authorSidhu, Ranjinder S.en_US
dc.date.accessioned2007-05-17T12:33:28Z
dc.date.available2007-05-17T12:33:28Z
dc.date.issued1998-09-01T00:00:00Zen_US
dc.identifier.urihttp://hdl.handle.net/1993/1351
dc.description.abstractIn the present study, we sought to investigate the age dependence of apoptosis during cerebral hypoxia-ischemia and the effectiveness of a specific caspase-3 inhibitor. To determine the role of apoptosis in mature and immature brain following an episode of cerebral hypoxia-ischemia, cell types were examined under light microscopy and were quantified morphologically. Evidence of punctate chromatin condensation, indicative of apoptosis, was greater in immature brain than in older brain. Role of caspase-3 was examined by measuring caspase-3 activity and caspase-3 protein using Western blotting techniques. Caspase-3 activity was significantly increased in animals exposed to hypoxia-ischemia, with a 3-fold greater caspase-3 activity in immature than older brain. In addition active caspase-3 was detectable 18hr post-hypoxia in 4 wk olds and at 4hr and 18hr following hypoxia in 1 wk olds. In the last study, animals were treated with a caspase-3 specific inhibitor in order to determine its effectiveness as a neuroprotective agent. Doses of 1.5-6$\mu$g/g body weight of z-DEVD-fmk I.P. were not effective in reducing infarction. (Abstract shortened by UMI.)en_US
dc.format.extent2629515 bytes
dc.format.extent184 bytes
dc.format.mimetypeapplication/pdf
dc.format.mimetypetext/plain
dc.language.isoengen_US
dc.rightsinfo:eu-repo/semantics/openAccess
dc.titleRole of apoptosis following cerebral hypoxia-ischemia in immature and older ratsen_US
dc.typeinfo:eu-repo/semantics/masterThesis
dc.typemaster thesisen_US
dc.degree.disciplinePharmacology and Therapeuticsen_US
dc.degree.levelMaster of Science (M.Sc.)en_US


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