Show simple item record

dc.contributor.authorFriesen, Mark Henryen_US
dc.date.accessioned2007-05-15T15:25:58Z
dc.date.available2007-05-15T15:25:58Z
dc.date.issued1997-04-01T00:00:00Zen_US
dc.identifier.urihttp://hdl.handle.net/1993/1010
dc.description.abstractLY303366 (LY) is an investigational echinocandin that has demonstrated activity against Candida species. We attempted to isolate an LY resistant mutant by plating the C. albicans clinical strain Y41 at a concentration of 10$\sp7$ CFU/plate on Brain Heart Infusion agar (BHI) plates supplemented with LY at concentrations of 1 to 40 $\mu$g/ml. After 7 days of incubation at 35$\sp\circ$C very small colonies were observed. One colony was selected and repeatedly subcultured on BHI with 1 $\mu$g/ml LY. After 5 passages the MIC was demonstrated to be 1.28 $\mu$g/ml LY, (wild type MIC = 0.04 $\mu$g/ml, an increase of 32$\times$). However, when 6 colonies were individually subcultured to Sabouraud dextrose agar (SDA) plates, only 3 remained resistant to LY. One of the transient mutant strains was evaluated in terms of: antifungal susceptibility (amphotericin B, 5-flucytosine, fluconazole, ketoconazole), growth, stability, and pharmacodynamic time-kill curves. There were no differences observed between parent and transient mutant strains in antifungal susceptibility, or growth patterns. Transient resistant strains reverted back to the parent MIC after 3 passages on SDA. The resistance phenotype was restored when this strain was cultured on BHI with 1 $\mu$g/ml LY. The transient resistant strain exhibit regrowth during pharmacodynamic time-kill curves at 1$\times$ MIC of LY after this strain had reverted to the wild type MIC. One of the stable mutant strains was evaluated in terms of: antifungal susceptibility (amphotericin B, 5-flucytosine, fluconazole, ketoconazole), morphology, growth, stability, pharmacodynamic time kill-curves, and germ tube formation. (Abstract shortened by UMI.)en_US
dc.format.extent7216387 bytes
dc.format.extent184 bytes
dc.format.mimetypeapplication/pdf
dc.format.mimetypetext/plain
dc.language.isoengen_US
dc.rightsinfo:eu-repo/semantics/openAccess
dc.titleIsolation and characterization of echinocandin resistant Candida albicans mutantsen_US
dc.typeinfo:eu-repo/semantics/masterThesis
dc.typemaster thesisen_US
dc.degree.disciplinePharmacyen_US
dc.degree.levelMaster of Science (M.Sc.)en_US


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record