Supplementary Table S3: Annotation of carbohydrate-active enzymes in C. stercorarium DSM8532T and coverage by MS/MS peptides Locus Product % peptide coverage (1) Modules (2) CAZy ID (3) Similar to NCBI Accession BLAST % similarity Clst_0021 phosphorylase 34 GH112 468861 Clst_0064 glycosyltransferase 19 GT4 468758 Clst_0091 glycoside hydrolase 4 GH95 468805 Clst_0092 alpha-L-fucosidase 28 GH29 468818 Clst_0098 amylase 1 CBM48-GH13 468783 Clst_0124 glycosyltransferase 1 GT2 468833 Clst_0138 glycosyltransferase 39 GT4 468776 Clst_0143 mannosyltransferase 30 GT32 468760 Clst_0153 beta-galactosidase 5 GH2 468780 Clst_0166 PelA 2 PL9 468817 AB106865.1 99% Clst_0206 xylan-binding protein 9 SLH(x3)-CBM54 468769 Clst_0224 glycosyltransferase 0 GT2 468859 Clst_0231 beta-galactosidase 6 GH2 468856 Clst_0237 BxlB 20 GH3 468774 AJ508405.1 99% Clst_0252 glycosyltransferase 0 GT2 468837 Clst_0253 arabinofuranosidase 0 GH127 468846 Clst_0254 amylase 0 GH15 468757 Clst_0295 BglZ 51 GH3 468862 Z94045.1 98% Clst_0308 beta-glucosidase 22 GH3 468773 Clst_0350 chitinase 4 CBM50(x2)-GH18 468787 Clst_0361 CepA 14 GH94 468786 U56424.1 100% Clst_0402 LysM domain-containing protein 2 CBM50 468756 Clst_0423 alpha-galactosidase 43 GH36 468771 Clst_0469 beta-1-4-xylanase 5 GH10 468755 Clst_0474 XylB 17 GH43 468754 AB106866.1 99% Clst_0520 deacetylase 0 CE4 468785 Clst_0567 carbohydrate-binding domain-containing protein 0 CBM25 468822 Clst_0576 ArfB 45 GH51 468795 AF002664.1 99% Clst_0578 rhamnogalacturonan lyase 0 PL11-SLH(x3) 468840 Clst_0579 rhamnogalacturonan lyase 0 PL11 468790 Clst_0588 beta-galactosidase 33 GH2 468858 Clst_0589 glycogen synthase 0 GT5 468813 Clst_0630 XynC 9 CBM22(x2)-GH10- CBM9-SLH 468800 AJ508408.1 99% Clst_0675 XynB 2 GH10 468763 D12504.1 99% Clst_0676 beta-xylosidase 0 GH43(x2)-CBM6 468759 Clst_0716 beta-1-4-xylanase 23 GH10 468854 Clst_0740 glycosyltransferase 3 GT4 468836 Clst_0774 transferase 23 GT28 468844 Clst_0794 endopolygalacturonase 21 GH28 468834 Clst_0804 deacetylase 11 CE4 468801 Clst_0811 alpha-galactosidase 23 GH4 468819 Clst_0851 XylA 29 GH43 468830 D13268.1 99% Clst_0856 BxlA 54 GH39 468823 AJ508404.1 99% Clst_0858 rhamnogalacturonase 0 GH105 468772 Clst_0890 maltodextrin phosphorylase 4 GT35 468814 Clst_0907 ArfA 18 GH43 468827 AJ508406.1 99% Clst_0908 rhamnogalacturonase 25 GH105 468860 Clst_0909 endopolygalacturonase 33 GH28 468850 Clst_0926 murein transglycosylase 8 GH23 468839 Clst_0928 glucuronidase 17 GH115 468799 Clst_0937 amylase 3 GH13 468778 Clst_0938 alpha-glucan phosphorylase 14 GT35 468810 Clst_0939 amylase 3 CBM34-GH13 468852 Clst_0942 beta-galactosidase 29 GH2 468770 Clst_0990 alpha-mannosidase 2 GH38 468863 Clst_0996 rhamnogalacturonase 0 GH105 468793 Clst_1001 rhamnogalacturonase 18 GH105 468812 Clst_1002 deacetylase 24 CE12 468781 Clst_1003 rhamnogalacturonase 4 GH105 468782 Clst_1016 RamA 5 GH78 468777 AJ238748.1 99% Clst_1066 beta-xylosidase 0 GH43 468838 Clst_1067 beta-xylosidase 6 GH43 468826 Clst_1086 xylanase 0 CBM35-GH26 468809 Clst_1087 mannosylglucose phosphorylase 15 GH130 468788 Clst_1124 LysM domain-containing protein 0 CBM50(x2) 468849 Clst_1168 beta-glucosidase 14 GH3-CBM6 468876 Clst_1171 alpha-glucosidase 4 GH31 468857 Clst_1301 beta-glucosidase 6 GH3 468835 Clst_1341 deacetylase 5 CE4 468784 Clst_1342 galactosidase 51 GH2 468802 Clst_1372 glycosyltransferase 19 GT2 468808 Clst_1394 deacetylase 59 CE7 468791 Clst_1455 LysM domain-containing protein 4 CBM50 468761 Clst_1564 rhamnogalacturonan lyase 12 PL11 468806 Clst_1586 AgaA 2 GH36 468824 AB089353.1 99% Clst_1627 alpha-galactosidase 29 GH27 468768 Clst_1647 endo-1,4-beta-galactosidase 3 CBM61(x3)-GH53- CBM61-SLH(x3) 468843 Clst_1660 galacturonate lyase 11 PL22 468765 Clst_1662 chitinase 5 SLH-SLH-SLH-GH18 468848 Clst_1666 murein transglycosylase 25 GH23 468864 Clst_1669 glycosyltransferase 2 GT4 468792 Clst_1783 pullulanase 6 GH13-CBM41(x2)- CBM48-GH13 468798 Clst_1823 CelZ 3 GH9-CBM3(x2) 468815 Z69359.1 99% Clst_1824 CelY 0 GH48-CBM3 468842 Z69359.1 99% Clst_1852 XynA 0 GH11-CBM6(x3) 468797 AJ508403.1 99% Clst_1911 N-acetylglucosamine-6-phosphate deacetylase 14 CE9 468865 Clst_2016 murein polymerase 34 GT51 468789 Clst_2100 CepB 51 GH94 468794 U60580.1 99% Clst_2112 glycosyltransferase 0 GT5 468766 Clst_2115 galactosidase 0 GH2 468775 Clst_2116 galactosidase 0 GH2 468831 Clst_2162 maltooligosyl trehalose synthase 54 GH13 468811 Clst_2164 deacetylase 44 CE1 468796 Clst_2177 beta-galactosidase 4 GH35 468779 Clst_2259 deacetylase 3 CE4 468820 Clst_2292 alpha-glucuronidase 42 GH67 468841 Clst_2338 CsaB 15 GT4 468807 Clst_2342 glycosyltransferase 37 GT4 468845 Clst_2367 chitinase 21 GH18 468821 Clst_2388 xylan-binding protein 1 SLH(x3)-CBM54 468762 Clst_2389 glucuronidase 21 GH88 468803 Clst_2401 N-acetylmannosaminyltransferase 19 GT26 468816 Clst_2440 beta-xylosidase 22 GH43 468851 Clst_2537 pectin methylesterase 21 CE8 468855 Clst_2631 N-acetylglucosamine-6-phosphate deacetylase 10 CE9 468853 (1) percent coverage of gene by MS/MS peptides (2) Modular structure of enzyme: GH = glycoside hydrolase, GT = glycosyltransferase, CE = carbohydrate esterase, PL = polysaccharide lyase, CBM = carbohydrate-binding module, SLH = surface layer homology domain, (3) Identification number in the Carbohydrate Active Enzymes (CAZy) database (www.cazy.org)