Hepatoprotective role of liver fatty acid binding protein in acetaminophen induced toxicity

Simple item page
dc.contributor.authorGong, Yu
dc.contributor.authorWang, Guqi
dc.contributor.authorGong, Yuewen
dc.contributor.authorYan, Jing
dc.contributor.authorChen, Yufei
dc.contributor.authorBurczynski, Frank J
dc.date.accessioned2014-04-04T06:57:11Z
dc.date.available2014-04-04T06:57:11Z
dc.date.issued2014-03-10
dc.date.updated2014-04-04T06:57:15Z
dc.description.abstractAbstract Background FABP1 has been reported to possess strong antioxidant properties. Upon successful transfection of the Chang cell line, which has undetectable FABP1 mRNA levels, with human FABP1 cDNA, the Chang cells were shown to express FABP1. Using the transfected and control (normal) Chang cells and subjecting them to oxidative stress, transfected cells were reported to be associated with enhanced cell viability. This study extends those observations by investigating the effect of FABP1 on acetaminophen (AAP)-induced hepatotoxicity. We hypothesized that presence of FABP1 would enhance cell viability compared to control cells (vector transfected cells). Methods Following AAP treatment of Chang FABP1 transfected and control cells, cell viability, oxidative stress, and apoptosis were evaluated using lactate dehydrogenase (LDH) release, the fluorescent probe DCF, and Bax expression, respectively. Results FABP1 cDNA transfected cells showed greater resistance against AAP toxicity than vector transfected cells. Significantly lower LDH levels (p < 0.05) were observed as were lower DCF fluorescence intensity (p < 0.05) in FABP1 cDNA transfected cells compared to vector transfected cells. FABP1 expression also attenuated the expression of Bax following AAP induced toxicity. Conclusion FABP1 attenuated AAP-induced toxicity and may be considered a cytoprotective agent in this in vitro model of drug induced oxidative stress.
dc.description.versionPeer Reviewed
dc.identifier.citationBMC Gastroenterology. 2014 Mar 10;14(1):44
dc.identifier.doihttp://dx.doi.org/10.1186/1471-230X-14-44
dc.identifier.urihttp://hdl.handle.net/1993/23397
dc.language.rfc3066en
dc.rightsopen accessen_US
dc.rights.holderYu Gong et al.; licensee BioMed Central Ltd.
dc.titleHepatoprotective role of liver fatty acid binding protein in acetaminophen induced toxicity
dc.typeJournal Article
Files
Original bundle
Now showing 1 - 2 of 2
Thumbnail Image
Name:
1471-230X-14-44.xml
Size:
50.56 KB
Format:
Extensible Markup Language
Description:
Download
Thumbnail Image
Name:
1471-230X-14-44.pdf
Size:
350.5 KB
Format:
Adobe Portable Document Format
Description:
Download
License bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
license.txt
Size:
2.17 KB
Format:
Item-specific license agreed to upon submission
Description:
Download
Collections
University of Manitoba Scholarship
Rady Faculty of Health Sciences Scholarly Works