TILRR Promotes Migration of Immune Cells Through Induction of Soluble Inflammatory Mediators

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Date
2020-07-03
Authors
Kashem, Mohammad
Ren, Xiaoou
Li, Hongzhao
Liang, Binhua
Li, Lin
Lin, Francis
Plummer, Francis
Luo, Ma
Journal Title
Journal ISSN
Volume Title
Publisher
Frontiers
Abstract
TILRR has been identified as an important modulator of inflammatory responses. It is associated with NF-kB activation and inflammation. Our previous study showed that TILRR significantly increased the expression of many innate immune responsive genes and increased the production of several pro-inflammatory cytokines/chemokines by cervical epithelial cells. In this study, we evaluated the effect of TILRR-induced proinflammatory cytokines/chemokines on the migration of immune cells. The effect of culture supernatants of TILRR-overexpressed cervical epithelial cells on the migration of THP-1 monocytes and MOLT-4 T-lymphocytes was evaluated using Transwell assay and a novel microfluidic device. We showed that the culture supernatants of TILRR-overexpressed HeLa cells attracted significantly more THP-1 cells (11–40%, p = 0.0004–0.0373) and MOLT-4 cells (14–17%, p = 0.0010–0.0225) than that of controls. The microfluidic device-recorded image analysis showed a significantly higher amount with a longer mean cell migration distance of THP-1 (p < 0.0001–0.0180) and MOLT-4 (p < 0.0001–0.0025) cells was observed toward the supernatants of TILRR-overexpressed cervical epithelial cells compared to that of the controls. Thus, the cytokines/chemokines secreted by the TILRR-overexpressed cervical epithelial cells attracted immune cells, such as monocytes and T cells, and may potentially influence immune cell infiltration in tissues.
Description
Keywords
TILRR, pro-inflammatory cytokines/chemokines, cervical epithelial cell culture supernatants, THP-1, MOLT-4, Transwell assay, microfluidic device
Citation
Kashem MA, Ren X, Li H, Liang B, Li L, Lin F, Plummer FA and Luo M (2020) TILRR Promotes Migration of Immune Cells Through Induction of Soluble Inflammatory Mediators. Front. Cell Dev. Biol. 8:563. doi: 10.3389/fcell.2020.00563