College of Dentistry - B.Sc. (Dent) Projects

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Browsing College of Dentistry - B.Sc. (Dent) Projects by Subject "Plasma Protein Interference"

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    Plasma Protein Interference at the Dentin-Adhesive Interface
    (2020-05-06) Bath, Dr. Karmvir; Franca, Dr. Rodrigo; Fiuza, Dr. Cristina
    Objective: The purpose of this study is to identify and confirm the presence of macromolecules and their mechanism that interferes with the dentin-adhesive interface. Despite, several advancements of adhesive systems, the bonded interface remains the weakest area of tooth-coloured restorations”. Our goal is to explore the use of chemical analysis via Fourier Transformed Infra-Red (FTIR) to identify bonds formed between adhesive and dentinal fluid protein. Materials & Methods: The protein solutions prepared in this experiment were the Albumin solution (45mg/mL dilution) and IgG solution (25mg/mL dilution) via 0.01M phosphate buffer solution creating constant concentrations as per human plasma. The adhesive systems used in this experiment were Elekta (BisGMA), All- Bond (MDP), Clear-Fill (MDP+MPDP). The experimentation concentrated on the three adhesive types (cured before contact) and then placed in time conditioned contact with protein solution for interactions of instant, 1min, 2min, and 5min. Chemical analysis was completed via FTIR Analysis Infrared spectra (FTIR) using a Thermo Scientific Nicolet™ 6700; in order to understand and identify the bonds formed between adhesive and dentinal fluid protein. Results: Our results show that albumin was detected after 2 minutes in all SEA samples, and IgG after 1 min for ClearFill, 2min for Elekta, and 5 min for Allbond. The detection identifies that a protein coating formation exists on the adhesive surfaces over time, which validates our hypothesis. Conclusion: Serum proteins react with the adhesive surface dependently on the adhesive composition.