Department of Biological Sciences

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Contributors to this community are faculty and students in the Department of Biological Sciences, Faculty of Science.

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Open Access
Lipids in Anadromous Northern Dolly Varden (Salvelinus malma malma)
(2023-03) Kulchycki, Lisa; Loseto, Lisa; Davoren, Gail
Anadromous Arctic fish species have adapted to a particular environment by evolving unique lipid cycling strategies such as storing large amounts of lipid during times of high productivity in order to survive long migrations, spawning events, and seasonal variation in food availability. Research on lipid content and storage location in the body is very limited, especially regarding the northern Dolly Varden, a fish species important culturally and for sustenance to the Indigenous Peoples in the western Canadian Arctic that is listed as ‘Special Concern’ under Species at Risk legislation. Lipid content in anadromous Dolly Varden obtained from two marine (coastal) (summer) and two freshwater (fall) locations were examined and compared to test for differences in percent lipid between locations/seasons. Percent lipid was compared between the muscle and homogenized whole-body of individuals caught in freshwater. Muscle lipid content was significantly different between freshwater locations and one of the marine locations (~34% higher from the marine location). One marine location contained fish with unexpectedly high muscle lipid percent. A weak/moderate linear relationship was found between lipid percent in the muscle tissue and whole-body tissue of the same individuals (r2= 0.2013 when sex was an added variable; r2= 0.4204 when reproductive status was an added variable), and reproductive status influenced this relationship. Sex of the individual did not affect lipid content in the muscle nor on the relationship between percent lipids in muscle and whole-body. Changing environmental factors due to climate change such as the timing of the ice melt and phytoplankton blooms can affect energy exchange through the food web, and thus research on the nature of fluctuating energy and lipid levels is needed to aid in conservation efforts of Arctic species.
Open Access
Individual timing consistency across purple martin (Progne subis) migrations
(2023-03) Bridges, Colin; Garroway, Colin (Biological Sciences); Davoren, Gail (Biological Sciences); Fraser, Kevin
Migration timing in long-distance migratory birds plays an essential role in individual survival and fitness. Migration schedules determine when individual birds depart and arrive seasonally between their overwintering and breeding sites. Bird migration timing may be largely driven by internal routines with some plasticity to environmental conditions, but individual timing across migrations has been little explored. To investigate the consistency of individual timing across migrations, I examined the individual order of migration timing in purple martins (Progne subis), a neotropical migratory songbird that travels between breeding sites throughout eastern North America and winter sites in Brazil. Migration timing data were collected for 295 different individual purple martins spanning over nine years by using light-level geolocators deployed during the breeding season at sites across the range and collected at the same sites the following year. I used a linear mixed-effect model (LMM) to examine the influence of the rank order of departure dates in one season on the rank order of four subsequent migration events while controlling for the effects of breeding latitude, sex, and age. Overall, I found that the individual rank order of migration timing in purple martins was conserved across migrations. Rank order timing was consistent between fall departure date from the breeding site and spring arrival dates in the following year (0.28  0.03, 95% CI 0.21-0.34), as well as the finer scale across fall migration departure and arrival dates (0.33  0.05, 95% CI 0.23-0.42), over the wintering period (0.39  0.04, 95% CI 0.30-0.48), and across spring migration (0.03  0.001, 95% CI 0.026-0.033). These results demonstrate that purple martin exhibit consistency in individual migration timing throughout the annual cycle. Migration distance also played a significant role, as the consistency of rank order timing lessened with distance traveled. Understanding how individual birds time migrations and if individuals are consistent between events can provide insight into how shifts in the environment with climate change could lead to a mismatch if migratory birds are unable to adapt. Future studies should examine if purple martins are able to adjust their migration timing and how long these changes persist in response to environmental alterations.
Open Access
Size matters: Host body mass overshadows climate change in parasite prevalence among semi-arid ground squirrels (Xerus inauris)
(2023-04) Guarino, Andrea; Davoren, Gail (Biological Sciences); Garroway, Colin (Biological Sciences); Waterman, Jane
Climate change is a phenomenon in which global temperatures are rising, and animals respond by undergoing thermal stress, which may be linked to increased susceptibility to ectoparasites. Environmental temperature greatly influences ectoparasites as they rely on external heat sources to regulate their body temperature. This study investigated the effects of increasing maximum and minimum temperatures on ectoparasites abundance (number of parasites on an individual) and prevalence (number of infected individuals) of adult female African Cape ground squirrels (Xerus inauris). This study also investigated the effects of host body mass and host body condition on ectoparasite abundance and prevalence. We did not find a relationship between rising temperatures and ectoparasite loads (abundance and prevalence), nor did we find a relationship between body condition and ectoparasite loads. We speculate that the thermoregulatory behaviours of Cape ground squirrels mitigate ectoparasite loads. We did not find evidence for a relationship between host body mass and abundance; however, we found a significant negative relationship between host body mass and parasite prevalence. Our results show that a higher proportion of individuals are infected with fleas compared to lice. It remains uncertain whether the inverse relationship between host body mass and prevalence is due to increased skin strength or other traits in larger individuals. The different life history traits of the parasites could explain why fleas are more prevalent than lice. Fleas, being facultative parasites, can avoid the grooming behaviour of hosts. In contrast, lice, being obligate parasites, are bound to remain on their host, thus, are more vulnerable to being removed. As climate change persists, the host-parasite relationship between Cape ground squirrels and ectoparasites may be worth revisiting.
Open Access
The importance of discs large homolog 5 and microRNA-34 expression to the development of the mosquito Aedes aegypti
(2023-04) Carroll, Lara; Wilkins, Olivia (Biological Sciences); Whyard, Steve
Understanding how the expression of particular genes and regulation of genes affects development in different organisms is key to advancing our knowledge in developmental biology. The epithelial-mesenchymal transition (EMT) process is commonly observed during the growth and development of various animal tissues. One gene involved in EMT regulation and directly related to development in humans is discs large homolog 5 (dlg5). If the role of this gene is conserved across species, it opens many medical application possibilities to help treat a multitude of medical issues, such as cancer. microRNAs are short, single-stranded non-coding RNAs that regulate gene expression by binding to the 3’UTR of complementary target mRNAs. microRNA-34 (miR-34) is a microRNA of interest with relation to dlg5 as it is predicted to bind with dlg transcripts. miR-34 has also shown clinical potential in the past with regulating cell proliferation. This study looks at the dlg5 gene and miR-34 in the mosquito Aedes aegypti to see if the expression of this gene and microRNA is evolutionarily conserved to that of Drosophila melanogaster. A developmental expression qRT-PCR analysis, analyzing the relative dlg and miR-34 transcript levels in different temporal stages of A. aegypti development, was conducted to compare the relative transcript levels of A. aegypti throughout development. Then, a dlgdsRNA RNAi-mediated knockdown by bacterial feeding was performed to see if knocking down dlg has an effect on development. The results of the qRT-PCR analysis and RNAi experiment were not significant enough to come to a justified conclusion. Therefore, further testing is required to produce meaningful conclusions regarding the developmental expression of dlg and miR-34.
Open Access
Comparing the Rates of Adaptive Change in Species Across Biogeographic Gradients
(2023-04) Bourrier-Vince, Ava; Fraser, Kevin (Biological Sciences); Jeffries, Kenneth (Biological Sciences); Garroway, Colin
The process of adaptive evolution allows a species to persist with specific traits tailored to their environment. The rate at which a population adapts can change based on the varying environmental abiotic and biotic factors of their location. Environmental characteristics such as the amount of available energy, elevation, temperature and degree of human disturbance thus affect the rates of adaptive evolution across biogeographic gradients. In this study, I used data on adaptive rates for terrestrial species and aimed to determine if the rates of adaptive evolution showed patterns related to spatial biogeographic patterns and environmental factors such as elevation, evapotranspiration, precipitation and temperature. Additionally, I tested whether the rates of adaption changed based on these specific environmental factors and a human density. The results of my data analysis detected that the rates of adaptation showed good variation in relation to spatial scale patterning but no significant correlation patterns between the tested environmental variables and the rates of adaptive evolution were detected. This result did not support my hypothesis that the rates of adaptive change would vary through space with environmental gradients. Many potential factors may have caused this inconclusive result. Further research into this topic would be useful to understand and predict the adaptive evolution of species’ in varying environmental gradients. I propose that studies on this data should aim to utilize more of the available samples within this public dataset such as the aquatic species. Additionally, further research may benefit from narrowing the study size based on location or taxa for significant relationships to be accurately detected.
Open Access
Sex-sorting Aedes aegypti for sterile insect technique (SIT) by knocking down four different gene targets, expressed in the guts of female larvae
(2023-04) Felix, Sharon
Mosquitoes are vectors for diseases that affect approximately 0.7 billion people globally. Most of these diseases have a high incidence in tropical often developing countries, where they prove either fatal or severely debilitating. Despite huge investments of time and money to find solutions, we have been unable to control this disease vector. Additionally, some of the techniques previously and currently in use to prevent these diseases, have serious drawbacks such as detrimental effects on non-target species, humans and the environment. We need eco-friendly alternatives to these harmful chemicals, that are just as efficient while also being species-specific to avoid harmful effects on other beneficial insect species. Sterile insect technique (SIT) is a creative technology that does just that. It involves the mass release of sterile males of a species to outcompete wild males and mate with wild females, eventually resulting in the decline of a targeted insect population. However, before these sterile males are released, they must be separated from females. An efficient way to sex-sort males from females can use RNA interference (RNAi). RNAi is a natural defence mechanism that exists in eukaryotic cells, that can be used to knock down female-biased or female-specific genes. Targeted females will develop slower than their male counterparts due to this knockdown and this will enable their separation from a pool of male individuals. For my honours thesis, I targeted four such female-biased genes - AAEL014604, AAEL014797, AAEL009313 and AAEL005884 and measured development (in days), survival and pupal size for each of these treatments for both male and female mosquitoes. None of my treatments resulted in significant results but one of these (AAEL014797) showed great promise for an improved SIT.
Open Access
Improving mosquito (Aedes aegypti) sex-sorting methods for sterile insect technique using RNAi gene knockdowns
(2023-04) Rempel, Kadri
Doublesex (dsx) is an alternatively spliced mosquito (Aedes aegypti) gene that controls sex development by producing male (DSXM) or female (DSXF) transcription factors that regulate gene expression in a sex-specific manner. As both DSXF and DSXM bind the same DNA sequence, their ability to regulate differential gene expression is predicted to be modulated by other proteins that interact with DSX. A previously conducted protein-protein interaction study identified several proteins that bind to DSXF and their role in altering female development was explored in this study by knocking down their corresponding transcripts using RNA interference during the larval stages of development. Knockdowns of the genes nop-14, wdr-48, and rnmt was achieved by feeding mosquito larvae HT115 strain E. coli transformed with a pl4440 plasmid containing a dsRNA template specific to each of three target genes. The knockdowns were carried out at their normal rearing temperature, 28oC, and at 22oC to slow mosquito development. Knockdown of one of the genes, wdr-48, at either temperature, significantly increased the time to pupation of females compared to males. This delayed development of females could prove useful in the sex sorting of males and females during the pupal stage by providing a method to select only males for a sterile male insect (SIT) population control program for Ae aegypti. Of note, the 22oC growth conditions provided a larger difference in the pupation times between the sexes than the 28oC conditions did, and thus may provide even better sex-sorting for mosquito SIT.
Open Access
Alternative BNIP3 splicing in alveolar rhabdomyosarcomal cells
(2023-04) Fernando, Amy; Whyard, Steve (Biological Sciences); Ghavami, Saeid (Human Anatomy and Cell Science); Gordon, Joseph; Wilkins, Olivia
Several cellular phenotypes drive tumorigenesis in alveolar rhabdomyosarcoma. These phenotypes may be attenuated via treatment with non-steroidal antiinflammatory drugs, like aspirin, tolfenamic acid, and indomethacin, which interfere with intracellular prostaglandin synthesis and induce cell death. Recent data suggest that this mechanism may be mediated by differential splicing of the cell death gene BNIP3, such that full-length BNIP3 could promote cell death, while short BNIP3 could inhibit it. Additional data indicate that oncogenic cytokines from the TGF-β family may also be involved in this mechanism. This honours thesis examines whether indomethacin alters cell death, BNIP3 splicing, and other alveolar rhabdomyosarcoma phenotypes. To assess this, RT-PCR and fluorescent imaging assays were performed on the RH30 cell line. Results of these experiments indicate that a 2.0 μM concentration of indomethacin alters the cell death phenotype. However, they also provide preliminary evidence that the expression levels of full-length and short BNIP3 are unchanged. Accordingly, drug treatments did not change calcium signalling pathways. Furthermore, this text examined the role of TGF-β cytokines in this molecular pathway. To help establish the role of TGF-β in alveolar rhabdomyosarcoma, a statistical analysis of a previously generated secretome dataset was performed. It determined that the three TGF-β isoforms are differentially secreted in alveolar and embryonal rhabdomyosarcoma. However, qRT-PCR results indicate that indomethacin exposure does not change TGF- β1 expression. Collectively, this thesis provides preliminary evidence that indomethacin exposure induces cell death in RH30s independently of alternative BNIP3 splicing.
Open Access
Evaluating different double-stranded RNA structures for their ability to control pest flea beetles
(2023-04) Verhaeghe, Lauren; Belmonte, Mark (Biological Sciences); Whyard, Steve
Canola is an economically important Canadian crop that suffers significant annual losses by damage from feeding flea beetles (Phyllotreta cruciferae and P. striolata). Increasing incidences of resistance and concerns about off-target effects with current insecticides demands new methods of control. Recently, environmentally safer approaches to pest control have been investigated using RNA interference (RNAi), a sequence-specific gene silencing mechanism triggered by exogenous double-stranded RNA (dsRNA), to selectively induce the mortality of targeted species. This study examined three different dsRNA structures for their ability to kill P. striolata flea beetles and thereby reduce feeding damage on canola leaves. Long linear dsRNAs (212-214 bp), short hairpin RNAs (hpRNAs) (21-24 bp), and short paperclip RNAs (pcRNAs) (21-24 bp) targeting mRNAs of three essential genes, Ras opposite (Rop), Sec23, and Snf7 in P. striolata were investigated. P. striolata adults were fed dsRNA-treated canola leaf disks, and impacts on insect survivorship and leaf material consumption were recorded over an eight-day period. pcRNAs targeting Sec23 and Snf7, and long dsRNAs targeting Sec23 and Rop effectively killed flea beetles, resulting in the reduced consumption of treated canola leaf tissues. Consumption of both the Sec23-specific long dsRNA and pcRNAs caused similar levels of flea beetle mortality (68% and 76%, respectively), whereas only the Rop-specific long dsRNA and the Snf7 pcRNA were effective at killing the beetles (76% and 84%, respectively). hpRNAs proved the least effective across all gene targets, killing at most, between 40-52% of flea beetles, depending on the gene target, and in general, these values were not significantly different relative to the negative controls. While this study examined dsRNAs specific for only three target genes, it provides evidence that RNAi-based pesticides have the potential to control these economically important pests and that short pcRNAs can be as effective as conventional long linear dsRNAs.
Open Access
Exploring the role of ammonia transporters (AMTs) in the branchial tissue of the horseshoe crab, Limulus polyphemus
(2023-05) Whiting, Holly; Campbell, Kevin (Biological Sciences); Treberg, Jason (Biological Sciences); Weihrauch, Dirk
All organisms must manage ammonia as it is highly toxic and a product of many essential biochemical processes. One group of proteins that facilitates the movement of ammonia across cell membranes is the Ammonia Transport Protein family which are generally sorted into three groups—ammonia transporters (AMTs), Rhesus glycoproteins (Rh proteins), and methylamine permeases (MEPs)—expressed in plants, animals, and fungi, respectively. Recently, transcripts of AMTs have also been found in invertebrates, where experimental evidence suggest that they play a role in both ammonia excretion and ammonia sensing. The American horseshoe crab, Limulus polyphemus, expresses at least two AMT and two Rh proteins within the epithelia of their book gills which is the primary surface for ammonia excretion. Each gill lamellae have a ventral ammonia permeable side and a dorsal ammonia impermeable side. mRNA transcripts for both proteins LpAMT-1 and LpAMT-3 were found on the dorsal and ventral surfaces, bringing into question their function in direct ammonia excretion. In oocyte expression trials both AMTs failed to mediate the transport of radiolabeled methylamine, while transport was detected for both a coral and a human Rh protein. Direct evidence of ammonia transport by invertebrate AMTs has not been previously found and further research should be conducted into the function of these proteins in invertebrates.
Open Access
Characterization of the oxygen binding properties of hemoglobin from the ruby-throated hummingbird (Archilochus colubris)
(2024-07-04) Patel, Suchita; Signore, Anthony (Biological Sciences); Fraser, Kevin (Biological Sciences); Campbell, Kevin
Weighing only between 2.5 and 4.8 g, the ruby-throated hummingbird (A. colubris) has one of the highest known mass-specific metabolic rates among birds. Despite this, there is a lack of available data on the oxygen binding properties of their hemoglobin (Hb). Here I measured the effect of allosteric effectors, pH and temperature on the O2-affinity (defined as the O2 partial pressure required for 50% Hb O2 saturation; P50) of ruby-throated hummingbird Hb, while also estimating their Hb buffering capacity. A comparison of Hb-O2 affinity (i.e. P50 values) between A. colubris and its sole congener, the black-chinned hummingbird (A. alexandri), revealed differences likely attributed to two β-chain amino acid substitutions. Under treatment conditions resembling the natural physiological state of hummingbird blood, A. colubris exhibited a similar Bohr effect (-0.401) and buffering capacity (4.88 mol H+ mol Hb4-1 pH-1) to those of other hummingbird species, though both variables were among the lowest values previously reported for birds. Nonetheless, the low buffering capacity is modelled to enhance the efficiency of their (relatively low) Bohr effect and may enable a high blood [Hb], which together with a relatively low blood O2 affinity is shown to markedly increase tissue O2 delivery. I also found that O2 binding of A. colubris Hb was thermally insensitive under natural physiological conditions. This thermal insensitivity could help ensure adequate O2 delivery both during and upon arising from torpor, while minimize heat loss at the lungs. These findings highlight the various O2 binding properties of A. colubris that together facilitate the efficient delivery of O2 to the tissues irrespective of temperature.
Open Access
An Investigation of Knowledge Retention Using Two-Stage Exams in Undergraduate Biology
(2024-07-04) Judge, Abby
Although active learning is typically thought of as an in-class experience, this concept can be further applied to assessments as well. Two-stage exams allow for the unique experience of students working collaboratively during examinations, following the completion of the individual stage. Although two-stage exams have been shown to improve student learning gains, the effects of two-stage exams on retention of course content remains variable. I examined the effects of two-stage exams on knowledge retention at various Bloom’s level (Remember, Understand, and Apply) to determine whether two-stage exams promote knowledge retention at various levels of cognitive thinking. A two-stage in-class test followed by the re-testing of questions answered individually or in groups at subsequent time points (5, 48, and 85 days) allowed testing for knowledge retention. Our results indicate that group questions improve knowledge retention at relatively long time periods across all Bloom’s levels while also promoting retention at more complex Bloom’s levels at intermediate and relatively long time periods. Additionally, our analysis reveals that an average of 40% of individual only questions are forgotten by the final exam, while only an average of 19.5% of group questions are forgotten by the final exam. These results indicate that two-stage exams promote the ability to retain complex information at relatively long time periods.
Open Access
Plant species-specific Effects on Boreal Soil Fertility in Mature and Fire-Disturbed Forest
(2024-07-04) Klapprat, Emily; Worley, Anne (Biological Sciences); Fraser, Kevin (Biological Sciences); Markham, John
Soil fertility is the ability for soil to meet the requirements for plant growth. The soil fertility is influenced by both fire disturbance and plant species. Competitive species contribute higher quality leaf litter than stress-tolerant species in the boreal forest which influences aspects of soil fertility. The effect that plants with different life history strategies have on soil fertility has not been studied in the context of high-severity fire. Soil fertility variables including organic layer depth, soil temperature, soil respiration rate, pH, inorganic nitrogen content, percent soil organic matter, and percent water retention were compared between soil samples from replicate plots in the boreal forest near Churchill Manitoba, and soil samples from replicate plots in a fire-disturbed site in the boreal forest. Additionally, soil fertility variables were compared between soils sampled from competitive species (Salix candida) and stress-tolerant species (Empetrum nigrum and Vaccinium uliginosum) growing in the two sites. Statistical differences in soil variables were found between soil samples from the boreal forest and soil samples from a fire-disturbed site. High-severity fire reduces the organic layer depth and decreases the pH, soil respiration rate, and available nutrients. Inorganic nitrogen content and relative growth rate of sea lyme grass grown in soil samples were statistically different between the competitive plant Salix candida and the stress-tolerant plants. Salix candida influences soil fertility by increasing the inorganic nitrogen content and fostering soils that increase the relative growth rate of sea lyme grass. As the frequency, intensity, and severity of fires in the boreal forest are increasing due to climate change, it is important to understand how species impact soil fertility.
Open Access
Quantifying parasitic swimbladder nematodes and their effect on the body condition of Lake Whitefish in Great Slave Lake
(2024-07-04) Friesen, Caitlyn
Parasites are known to impact the health of their hosts due in part to depleting available energy. Although Cystidicola farionis are common parasitic swimbladder nematodes of Lake Whitefish, it is difficult to ascertain how infection affects the host’s health. It is essential to understand parasite and host biology to assess the effects of parasitic infection on the host’s body condition. Given that of the Cystidicola species, only C. farionis has been identified in Lake Whitefish, it is predicted that the nematodes will be identified as such. Reproduction is a major driver of an individual’s health, and female Lake Whitefish invest more energy in reproductive events than males. The Fulton’s (K) condition index was used to assess somatic body condition, where the inclusion of gonad weight could potentially mask a sex-specific effect of parasite infection. The gonadosomatic index (GSI) was used to determine if C. farionis infection has a sex-specific effect on Lake Whitefish’s body condition. Swimbladder nematodes were identified based on egg morphology and genetic sequencing of the 28S and ITS-2 regions of the ribosomal DNA. For the first time in Great Slave Lake, C. farionis was identified based on morphological and genetic analysis, and prevalence was estimated at 54% in mature Lake Whitefish with a mean intensity of infection of 9.74 nematodes per infected host. T-tests indicated that body condition did not differ between Lake Whitefish infected with C. farionis and those uninfected. Although, linear regression models revealed that as the intensity of infection increased, K values of mature Lake Whitefish and GSI for current-year spawning females declined. There did not appear to be a sex-specific effect of infection, although future examination of current-year spawning males and immature Lake Whitefish could provide further insight into the impact of C. farionis infection.
Open Access
Self-Grooming Behaviors in the Maternal Immune Activation Mouse Model of Autism Spectrum Disorder
(2024-07-04) Ayoub, Manahil; Stacey, Joy (Biological Sciences); Xing, Lei (Biological Sciences); Balci, Fuat
Autism spectrum disorder (ASD) is a neurodevelopmental condition that effects approximately 1% of children worldwide. Hence, it is vital to develop animal models of ASD so that thorough investigation of the disorder’s contributing developmental factors and underlying etiology can take place. Mice remain one of the best animal models used in ASD research as they share genetic, physiological, and anatomical similarity to humans, of which transgenic strains can be used to represent neurodevelopmental disorders including ASD. Considering that most clinical cases of ASD are idiopathic in nature, where it is suspected that environmental conditions play a substantial role in the development of ASD in the fetus, it is important to develop and validate idiopathic mouse models of ASD. Ultimately, we hope these mouse models would provide insight on idiopathic manifestation of ASD that syndromic models might lack. The maternal immune activation (MIA) mouse model of ASD uses an inflammatory response in a pregnant mouse dam to induce ASD-like symptom in the pups. In our experiments, this was achieved using a poly-IC injection in the pregnant dam. In this study we look to further validate the MIA model of autism by carrying out an open-field test looking at the self-grooming behaviours of MIA autistic mice and comparing them to an unmanipulated control group. We used parameters in grooming behaviour that past open-field tests on syndromic models of ASD have shown deviation from control groups in terms of self-grooming behaviours. While our sample size was too small to establish significant differences, we established future corrections in the methodology of the open field self-grooming tests and provide suggestions on the improvement of their accuracy.
Open Access
Electrochemical Detection of Platinum-Based Chemoresistance and Correlation with MEK1 Activity in Living Cancer Cells
(2024-07-04) Daou, Roy; Whyhard, Steve (Biological Sciences); Doering, Jennifer (Biological Sciences); Lee, Jae-Hyeok (Biological Sciences); Kuss, Sabine
Drug resistance is responsible for most chemotherapy failures, making it an urgent issue in modern oncology. In ovarian cancer, resistance to platinum-based chemotherapeutics, such as carboplatin, is common. However, its underlying mechanisms remain poorly understood. A mitogen-activated protein kinase kinase, MEK1, may play a role in the development of this resistance mechanism. This project aims to understand this mechanism by using western blots to measure MEK1 activity in patient-obtained living ovarian cancer cells, comparing between carboplatin-susceptible and carboplatin-resistant cells. Moreover, early detection of chemoresistance can improve treatment outcomes. However, current detection methods are inefficient and time-consuming. To address this, the project also proposes an unconventional approach to identifying chemoresistance. This involves the use of scanning electrochemical microscopy for the rapid and accurate quantification of glutathione, a prominent antioxidant that may serve as a biomarker for platinum-based resistance mechanisms, in both cell lines. The findings suggest a correlation between lower MEK1 activity and increased glutathione levels in the resistant cells. This research may contribute to the development of an efficient and reliable method for detecting chemoresistance, with the potential of significantly improving cancer treatment outcomes.
Open Access
Arthropod Diversity Within The Three Sisters Cropping System
(2024-07-04) Ferch, Tessa; Roth, Jim (Biological Sciences); Markham, John (Biological Sciences); Bobiwash, Kyle; Worley, Anne
The Three Sisters is an indigenous cropping method that dates back hundreds of years. It is comprised of the simultaneous planting of corn, bean, squash, and sometimes sunflower. However, there has been no published research into arthropod communities and interactions with this cropping system. My study examined arthropod abundance and taxa to the family level at three areas: uncultivated, fields planted with The Three Sisters, and field edges. This was done at three sites in Manitoba: Ian N. Morrison Research Farm near Carman, Glenlea Research Station near Glenlea, and Brokenhead Ojibway First Nations Reserve. I placed 8 sticky cards within each area at each site starting in July 2023, and had 4 bi-weekly sampling rounds. The number of samples collected and processed was 288. I conducted a perMANOVA test, and ran multiple negative binomial generalized linear mixed models, testing the abundance and number of taxa present in relation to the site, area type, date, colour, and using trap number as a random effect. All sites were significantly different from each other. Glenlea had the highest diversity, and Carman had the least diversity. The uncultivated areas differed from both the field and edge areas, and the fields and edges also differed from each other at all sites. The field and edges had higher diversity than the uncultivated areas. The most important finding of my study was that The Three Sisters is an agricultural method that can allow for higher diversity of arthropods than uncultivated areas.
Open Access
Mixed-Grass Prairie Field Restoration: How long does it really take?
(2024-07-04) Farrant, Daryn; Zelmer, Carla (Biological Sciences); Fraser, Kevin (Biological Sciences); Koper, Nicola; Markham, John
This thesis explores the success of mixed-grass prairie field restoration efforts in Grassland National Park, SK (GNP) by analyzing the progress of several fields over time; Laouenan 2001, Belzas 2003 and Gergovia 2005, relative to GNP’s target endpoints. The surrounding vegetation is dominated by native grasses. In addition, I ran a space for time analysis on the Gergovia fields, where measured data from 2021 for fields of varying seeding, years was substituted for time. This allowed me to use data collected from the newer monitoring protocol to track changes in vegetation over time. Cover estimates of bare ground, litter, native herbaceous were tested against target endpoints chosen by GNP to reflect that of native prairie, extrapolated from Thorpe (2007), in order to determine if a field is considered restored. Based on the findings of this study we can conclude that GNP’s fields that met targets for bare ground took an average of 10 years to match that of native prairie. In all GNP’s fields the bare ground target took the longest to be met, with one field failing to meet the target 13 years after seeding. Furthermore, at GNP, native grasses took an average of 5 years to establish and reach the target endpoint. Litter in all fields were found to exceed that of Native prairie, indicating a need for a prescribed burn or other management strategies. The endpoint target not met (exceeding the target) 10 years post-seeding was non-native species which could be reduced using a prescribed burn. This study underscores the importance of comprehensive monitoring protocols, field initiation and seed mix documentation for evaluating restoration success accurately. Overall, the findings affirm the effectiveness of GNP's restoration efforts and emphasize the need for further research into species composition and diversity to fully meet the SER (2004) criteria of a restoration project, which includes the reparation of ecosystem processes, productivity and services, and the re-establishment of the pre-existing biotic integrity in terms of species composition and community structure (SER 2004).
Open Access
Stable Isotopes Reveal Primary Flight Feather Moult Patterns of Atlantic Puffins (Fratercula arctica)
(2024-07-04) Currie, Carolyn; Markham, John (Biological Sciences); Fraser, Kevin (Biological Sciences); Davoren, Gail
Feathers are essential for bird survival but constantly degrade, requiring regular replacement (i.e., moult). Although necessary, moult is energetically costly and has evolved to be temporally separated from other costly activities, such as breeding. Wing-propelled pursuit divers in the family Alcidae (‘alcids’) have high wing loading (body mass/wing area) and, thus, may become flightless when wing area is reduced during primary feather moult. Different primary moult patterns require different durations to complete, as some extend the moult period while others minimize it. Thus, depending on the moult pattern, it leaves them in a vulnerable state for different lengths of time. As alcids typically moult while offshore, little is known about the flight feather moult patterns of most species, including Atlantic Puffins (Fratercula arctica). This study examined whether the primary flight feather moult pattern of Atlantic Puffins is descendent (slow, sequential replacement of primary feathers from the innermost (P1) to the outermost (P10)) or catastrophic (near-simultaneous replacement of P1-P10) using stable isotope ratios of carbon (δ13C) and nitrogen (δ15N). Puffin carcasses (n = 25) were collected from James Island, Newfoundland, Canada during August 2020-2022 and differences in isotope ratios between P1-P2 were compared with differences between P1-P5, and P1-P10. A lack of differences in δ13C and δ15N across feather pairs suggest a catastrophic moult pattern; however, higher P1-P10 differences in some birds indicate these individuals may have a descendent moult pattern or may moult half of their primaries before breeding, and half after. These findings increase our understanding of moult patterns in alcids, which is important to understand vulnerable periods associated with primary feather moult.
Open Access
Investigating the Roles of Homeobox Proteins and TEAD in Small Cell Lung Cancer
(2024-07-05) Ricarte, Bea; Pearson, Joel
Lung cancer remains a significant global health concern, responsible for a substantial number of cancer-related deaths annually. Small cell lung cancer (SCLC), one of its subtypes, is particularly aggressive and resistant to treatment, leading to a poor prognosis for patients. Unfortunately, treatment options for SCLC remain limited and have not been advanced for decades. This highlights the urgent need for a deeper understanding of the molecular mechanisms underlying the pathogenesis of SCLC in order to identify novel therapeutic strategies for this disease. DNA-bind proteins, TEADs, play a crucial role in promoting SCLC survival. TEADs are best recognized for their role in interacting with transcriptional activators, YAP and TAZ, however, it was found that the function of TEADs in SCLC is completely independent of YAP and TAZ. Beyond this however, the mechanisms underlying the critical function of TEAD in SCLC is unknown. Preliminary data gathered by the Pearson lab identified the presence of homeobox proteins PROX1, NKX2-1 and NKX2-2 with the TEAD complex in SCLC, prompting the hypothesis that TEAD interacts with these proteins to silence TEAD target genes in SCLC, promoting the cancerous state. Using co-immunoprecipiation, the association of TEAD with PROX1 and NKX2-1 were confirmed. Additionally, CRISPR interference was used to knockdown PROX1, NKX2-1 and NKX2-2 in SCLC cells, to assess their role in TEAD-mediated gene regulation. This resulted in an increase in controlled cell regulation and differentiation genes, and a decrease in SCLC biomarkers, consistent with the hypothesis. However, contrasting the hypothesis, protein knockdowns showed a decrease of cell adhesion genes. Nevertheless, these findings bring light to a novel pathway in SCLC pathogenesis. Understanding the exact mechanisms behind these homeobox proteins and their interaction with TEADs could lead to the development of more effective treatment for SCLC.

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