SCI Stem Cells International 1687-9678 1687-966X Hindawi Publishing Corporation 10.1155/2016/7168175 7168175 Research Article Inhibition of Viability, Proliferation, Cytokines Secretion, Surface Antigen Expression, and Adipogenic and Osteogenic Differentiation of Adipose-Derived Stem Cells by Seven-Day Exposure to 0.5 T Static Magnetic Fields Wang Jian jianwang1030@126.com 1,2,3 2 3 Xiang Bo xiangb@cc.umanitoba.ca 2,4 4 Deng Jixian jixian.deng@nrc-cnrc.gc.ca 2,3 3 Freed Darren H. dhfreed@ualberta.ca 2,3,5 3 5 Arora Rakesh C. rarora@sbgh.mb.ca 2,3,6 3 6 Tian Ganghong ganghong.tian@nrc-cnrc.gc.ca 2,3 3 Sluijter Joost 1 Department of Vascular Surgery Union Hospital Tongji Medical College Huazhong University of Science and Technology 1277 Jiefang Street Wuhan Hubei 430022 China hust.edu.cn 2 National Research Council of Canada 435 Ellice Avenue Winnipeg MB Canada R3B 1Y6 nrc-cnrc.gc.ca 3 Department of Physiology Faculty of Medicine University of Manitoba 727 McDermot Avenue Winnipeg MB Canada R3E 3P5 umanitoba.ca 4 Department of Pharmacology and Therapeutics Faculty of Medicine University of Manitoba 753 McDermot Avenue Winnipeg MB Canada R3E 0T6 umanitoba.ca 5 Division of Cardiac Surgery University of Alberta Hospital 8440-112 Street Edmonton AB Canada T6G 2B7 ualberta.ca 6 Cardiac Science Program Institute of Cardiovascular Science St. Boniface General Hospital 409 Tache Avenue Winnipeg MB Canada R2H 2A6 saintboniface.ca 2016 6 1 2016 2016 07 09 2015 24 11 2015 26 11 2015 2016 Copyright © 2016 Jian Wang et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

After seven-day exposure to 0.5-Tesla Static Magnetic Field (SMF), Adipose-derived Stem Cells (ASCs) and those labeled by superparamagnetic iron oxide (SPIO) nanoparticles were examined for viability by methyl thiazol tetrazolium (MTT) assay, proliferation by cell counting and bromodeoxyuridine (BrdU) incorporation, DNA integrity by single cell gel electrophoresis, surface antigen by flow cytometry analysis, and the expression of cytokines and genetic markers by reverse transcription-PCR and underwent adipogenic and osteogenic differentiation assessed by quantifying related specific genes expression. The SMF slightly reduced cell viability and proliferation and inhibited the expression of CD49d, CD54, and CD73 but did not damage DNA integrity. The SMF slightly downregulated the expression of cytokines including Vascular Endothelial Growth Factor (VEGF), Insulin-like Growth Factor-1 (IGF-1), Transforming Growth Factor Beta 1 (TGF-β1), genetic markers comprising Stem Cell Antigen-1 (Sca1), Octamer-4 (Oct-4), ATP-binding Cassette Subfamily B Member 1 (ABCB1), adipogenic marker genes containing Lipoprotein Lipase (LPL), Peroxisome Proliferator-Activated Receptor Gamma (PPAR-γ), and osteogenic marker genes including Secreted Phosphor-protein 1 (SPP1) and Osterix (OSX). Exposure to 0.5 T SMF for seven days inhibited viability, proliferation, surface antigen expression, cytokine secretion, stem cell genetic marker expression, and adipogenic and osteogenic differentiation but did not affect the DNA integrity in ASCs with or without SPIO labeling.