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Title: Role of liver fatty acid binding protein in fatty liver cell culture model
Authors: Chen, Yufei
Supervisor: Burczynski, Frank J (Pharmacy)
Examining Committee: Gong, Yuewen (Pharmacy) Minuk, Gerald Y (Pharmacology and Therapeutics)
Graduation Date: May 2012
Keywords: L-FABP
antioxidant therapy
oxidative stress
Issue Date: 5-Apr-2012
Abstract: Liver fatty acid binding protein has been reported to possess antioxidant properties in the liver. The aim of this study was to investigate the effect of this protein in a nonalcoholic fatty liver disease (NAFLD) cell culture model. Rat hepatoma cells were treated with an oleate:palmitate (2:1) mixture for either 1 and 2 days, or further treated with 500 µM clofibrate to induce L-FABP expression. Intracellular lipid accumulation was quantitated by Nile Red. Lipotoxicity was determined using the WST-1 assay. Dichlorofluorescein (DCF) was utilized to assess intracellular reactive oxidative species (ROS) level. Measurement of lipotoxicity showed statistical decreases in cell viability as lipid concentrations increased in a dose-dependent manner. NAFLD cell cultures showed characteristic cellular damage from increased ROS levels in fatty acid treated cells. All groups treated with clofibrate showed statistically increased intracellular L-FABP levels and reduced ROS levels. The results lead to the conclusion that clofibrate induces L-FABP expression and in this manner suppresses hepatocellular ROS generation.
Appears in Collection(s):FGS - Electronic Theses & Dissertations (Public)

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