CD26/ dipeptidyl peptidase IV (CD26/DPPIV) is highly expressed in peripheral blood of HIV-1 exposed uninfected Female sex workers

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dc.contributor.authorSongok, Elijah M
dc.contributor.authorOsero, Bernard
dc.contributor.authorMckinnon, Lyle
dc.contributor.authorRono, Martin K
dc.contributor.authorApidi, Winnie
dc.contributor.authorMatey, Elizabeth J
dc.contributor.authorMeyers, Adrienne FA
dc.contributor.authorLuo, Ma
dc.contributor.authorKimani, Joshua
dc.contributor.authorWachihi, Charles
dc.contributor.authorBall, Blake T
dc.contributor.authorPlummer, Frank A
dc.contributor.authorMpoke, Solomon
dc.date.accessioned2013-03-15T08:08:26Z
dc.date.available2013-03-15T08:08:26Z
dc.date.issued2010-11-25
dc.date.updated2013-03-15T08:08:27Z
dc.description.abstractAbstract Background Design of effective vaccines against the human immunodeficiency virus (HIV-1) continues to present formidable challenges. However, individuals who are exposed HIV-1 but do not get infected may reveal correlates of protection that may inform on effective vaccine design. A preliminary gene expression analysis of HIV resistant female sex workers (HIV-R) suggested a high expression CD26/DPPIV gene. Previous studies have indicated an anti-HIV effect of high CD26/DPPIV expressing cells in vitro. Similarly, high CD26/DPPIV protein levels in vivo have been shown to be a risk factor for type 2 diabetes. We carried out a study to confirm if the high CD26/DPPIV gene expression among the HIV-R were concordant with high blood protein levels and its correlation with clinical type 2 diabetes and other perturbations in the insulin signaling pathway. Results A quantitative CD26/DPPIV plasma analysis from 100 HIV-R, 100 HIV infected (HIV +) and 100 HIV negative controls (HIV Neg) showed a significantly elevated CD26/DPPIV concentration among the HIV-R group (mean 1315 ng/ml) than the HIV Neg (910 ng/ml) and HIV + (870 ng/ml, p < 0.001). Similarly a FACs analysis of cell associated DPPIV (CD26) revealed a higher CD26/DPPIV expression on CD4+ T-cells derived from HIV-R than from the HIV+ (90.30% vs 80.90 p = 0.002) and HIV Neg controls (90.30% vs 82.30 p < 0.001) respectively. A further comparison of the mean fluorescent intensity (MFI) of CD26/DPPIV expression showed a higher DPP4 MFI on HIV-R CD4+ T cells (median 118 vs 91 for HIV-Neg, p = 0.0003). An evaluation for hyperglycemia, did not confirm Type 2 diabetes but an impaired fasting glucose condition (5.775 mmol/L). A follow-up quantitative PCR analysis of the insulin signaling pathway genes showed a down expression of NFκB, a central mediator of the immune response and activator of HIV-1 transcription. Conclusion HIV resistant sex workers have a high expression of CD26/DPPIV in tandem with lowered immune activation markers. This may suggest a novel role for CD26/DPPIV in protection against HIV infection in vivo.
dc.description.versionPeer Reviewed
dc.identifier.citationVirology Journal. 2010 Nov 25;7(1):343
dc.identifier.doihttp://dx.doi.org/10.1186/1743-422X-7-343
dc.identifier.urihttp://hdl.handle.net/1993/17605
dc.language.rfc3066en
dc.rightsopen accessen_US
dc.rights.holderElijah M Songok et al.; licensee BioMed Central Ltd.
dc.titleCD26/ dipeptidyl peptidase IV (CD26/DPPIV) is highly expressed in peripheral blood of HIV-1 exposed uninfected Female sex workers
dc.typeJournal Article
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