HasF, a TolC-homolog of Serratia marcescens, is involved in energy-dependent efflux

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dc.contributor.authorKumar, A
dc.contributor.authorWorobec, EA
dc.date.accessioned2007-10-10T18:17:00Z
dc.date.available2007-10-10T18:17:00Z
dc.date.issued2005-06-30
dc.description.abstractA tolC-like gene (hasF) was identified upon scanning the incomplete database of the S. marcescens genome. This gene was amplified using PCR and cloned in the pUC18 vector to yield pUCHF. Sequencing of the S. marcescens tolC-like hasF gene and subsequent amino acid sequence prediction revealed approximately 80% amino acid homology with the Escherichia coli TolC. A tolC-deficient strain of E. coli (BL923) containing pUCHF/hasF was analyzed for susceptibility to fluoroquinolones (ciprofloxacin, norfloxacin, and ofloxacin), chloramphenicol, sodium dodecyl sulfate (SDS), and ethidium bromide. Antibiotic susceptibility assays of the E. coli tolC-deficient mutant BL923 demonstrated a 64-fold increase in resistance to SDS and ethidium bromide upon introduction of the S. marcescens tolC-like hasF gene. No change was observed for susceptibility to fluoroquinolones and chloramphenicol. Ethidium bromide accumulation assays performed using E. coli BL923:pUCHF established the role of the S. marcescens hasF gene product in proton gradient-dependent efflux.en
dc.format.extent49240 bytes
dc.format.mimetypeapplication/pdf
dc.identifier.citation0008-4166; CAN J MICROBIOL, JUN 2005, vol. 51, no. 6, p.497 to 500.en
dc.identifier.doihttp://dx.doi.org/10.1139/w05-029
dc.identifier.urihttp://hdl.handle.net/1993/2930
dc.language.isoengen_US
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dc.rightsopen accessen_US
dc.statusPeer revieweden
dc.subjectHasFen
dc.subjectTolCen
dc.subjecteffluxen
dc.subjectS. marcescensen
dc.subjectMEMBRANE PROTEINen
dc.subjectMULTIDRUG EFFLUXen
dc.subjectRESISTANCEen
dc.subjectCLONINGen
dc.subjectEXPORTen
dc.subjectPUMPen
dc.titleHasF, a TolC-homolog of Serratia marcescens, is involved in energy-dependent effluxen
dc.typejournal articleen_US
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