Sequence and chromosomal assignment of a human novel cDNA: similarity to gamma-aminobutyric acid transporter

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dc.contributor.authorGong, YW
dc.contributor.authorZhang, MN
dc.contributor.authorCui, L
dc.contributor.authorMinuk, GY
dc.date.accessioned2007-10-10T18:59:11Z
dc.date.available2007-10-10T18:59:11Z
dc.date.issued2001-12-31
dc.description.abstractGamma-aminobutyric acid (GABA) is the predominant inhibitory neurotransmitter in the mammalian brain. Although initially thought to be confined to the central nervous system, GABAergic activity has also been described in other tissues throughout the body. In the present study, we report the cloning and localization of human GABA transporter cDNA and document its expression in various human tissues. A human liver cDNA library was initially screened by a P-32-labeled murine brain GABA transporter 3 (GAT-3) cDNA probe, and full-length cDNA was cloned by employing Marathon-Ready(TM) human kidney cDNA. The human GABA transporter cDNA encoded a 569 amino acid hydrophobic protein with 12 transmembrane domains (TMs). Search of published sequences revealed high homology with rat GAT-2, murine GAT-3 cDNA, human solute carrier family 6 member 13 (SLC6A13), and a human peripheral betaine/GABA transporter. Northern blot analyses demonstrated that the human GABA transporter is expressed strongly in the kidney and to a lesser extent in the liver and brain. The sequence was well matched with human chromosome 12p13.3, suggesting the human GABA transporter contains 14 exons. The above findings confirm the existence of and further characterize a specific GABA transporter in human tissues.en
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dc.identifier.citation0008-4212; CAN J PHYSIOL PHARMACOL, DEC 2001, vol. 79, no. 12, p.977 to 984.en
dc.identifier.urihttp://hdl.handle.net/1993/2938
dc.language.isoengen_US
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dc.rightsopen accessen_US
dc.statusPeer revieweden
dc.subjectsequenceen
dc.subjectchromosomeen
dc.subjectGABAen
dc.subjectGABA transporteren
dc.subjectHEPATIC REGENERATIVE ACTIVITYen
dc.subjectGABA LEVELSen
dc.subjectRAT-BRAINen
dc.subjectENCEPHALOPATHYen
dc.subjectCLONINGen
dc.subjectSYSTEMen
dc.subjectEXPRESSIONen
dc.subjectPLASMAen
dc.subjectDISEASEen
dc.subjectBINDINGen
dc.titleSequence and chromosomal assignment of a human novel cDNA: similarity to gamma-aminobutyric acid transporteren
dc.typejournal articleen_US
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