Comparison of the rapid insulin sensitivity test (RIST), the insulin tolerance test (ITT), and the hyperinsulinemic euglycemic clamp (HIEC) to measure insulin action in rats

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dc.contributor.authorReid, MAG
dc.contributor.authorLatour, MG
dc.contributor.authorLegare, DJ
dc.contributor.authorRong, N
dc.contributor.authorLautt, WW
dc.date.accessioned2008-01-21T17:55:43Z
dc.date.available2008-01-21T17:55:43Z
dc.date.issued2002-08-31
dc.description.abstractThe objective was to compare the ability of the rapid insulin sensitivity test (RIST), the hyperinsulinemic euglycemic clamp (HIEC), and the insulin tolerance test (ITT) to detect hepatic insulin sensitizing substance (HISS) dependent insulin action. HISS action was augmented by feeding and inhibited by fasting, blockade of hepatic nitric oxide synthase, or blockade of hepatic muscarinic cholinergic receptors. A significant correlation was found between the RIST index and ITT nadir (r(2) = 0.84), but not between the glucose infusion rate of the HIEC and RIST index. There was, however, a relationship between the RIST index and the initial response during the HIEC. Use of the HIEC resulted in HISS-dependent insulin resistance in both conscious and anesthetized animals. We concluded that since the RIST and ITT were comparable in quantifying both HISS-dependent and HISS-independent insulin action, the RIST was validated against this standard. The observation that the HIEC is capable of detecting HISS action in the first rising slope of the test but not at the end of the test and that HISS release is fully blocked after the conclusion of the HIEC raises concerns about the use of the commonly used HIEC.en
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dc.identifier.citation0008-4212; CAN J PHYSIOL PHARMACOL, AUG 2002, vol. 80, no. 8, p.811 to 818.en
dc.identifier.doihttp://dx.doi.org/10.1139/y02-102
dc.identifier.urihttp://hdl.handle.net/1993/3007
dc.language.isoengen_US
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dc.rightsopen accessen_US
dc.statusPeer revieweden
dc.subjectHISSen
dc.subjectinsulin resistanceen
dc.subjectinsulin sensitivity testsen
dc.subjectCHOLINERGIC BLOCKADEen
dc.subjectGLUCOSEen
dc.subjectRESISTANCEen
dc.subjectREPRODUCIBILITYen
dc.subjectHUMANSen
dc.subjectOSCILLATIONSen
dc.subjectINDUCTIONen
dc.subjectATROPINEen
dc.titleComparison of the rapid insulin sensitivity test (RIST), the insulin tolerance test (ITT), and the hyperinsulinemic euglycemic clamp (HIEC) to measure insulin action in ratsen
dc.typejournal articleen_US
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