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Title: NIR imaging of vascular endothelial cells using Cy5.5-lectin conjugates
Authors: Nguyen, Cecilia
Supervisor: Jilkina, Olga (Oral Biology)
Examining Committee: Sowa, Mike (Oral Biology) Bhullar, Raj (Oral Biology) Arthur, Gilbert (Biochemistry and Medical Genetics)
Graduation Date: October 2012
Keywords: Imaging
Endothelial cells
Issue Date: 27-Feb-2012
Publisher: Journal of Biophotonics
Citation: Nguyen C, Bascaramurty S, Kuzio B, Gregorash L, Kupriyanov V, Jilkina O. 2012. Near-infrared fluorescence imaging of mouse myocardial microvascular endothelium using Cy5.5-lectin conjugate. Journal of Biophotonics (early view). <10.1002/jbio.201100119>.
Abstract: The objective of this study was to develop a fluorescent near-infrared endothelial cell binding conjugate using Lycopersicon esculentum lectin and Cy5.5 N-hydroxysuccinimide ester for the purpose of imaging the microvascular network in mouse hearts under in vivo and ex vivo conditions. Cy5.5-lectin conjugate was synthesized with a dye/protein ratio of 2.90 ± 1.54 (n=6). Mouse hearts were successfully labelled in both in vivo and ex vivo and showed similar labelling patterns. Cy5.5-lectin labelling patterns and that of ICAM2 and FITC-lectin co-localized, indicating binding to endothelial cells. Finally, it was shown that Cy5.5-lectin is capable of visualizing, in real-time, areas of normal and abnormal heart perfusion at resolutions of 76.8 pixels/mm. Areas of the heart that were not perfused post-ligation displayed no Cy5.5 staining on histological sections and during real-time cardiac imaging of intact hearts showed minimal fluorescent signal (~35 a.u.) compared to areas where normal perfusion occurred (~150 a.u.).
Appears in Collection(s):FGS - Electronic Theses & Dissertations (Public)

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