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|Title: ||Genetic characterization of Canadian group A human rotavirus strains collected in multiple paediatric hospitals from 2007-2010|
|Authors: ||McDermid, Andrew|
|Supervisor: ||Booth, Tim (Medical Microbiology)|
|Examining Committee: ||Embree, Joanne (Medical Microbiology) Coombs, Kevin (Medical Microbiology) Crockett, Maryanne (Medical Microbiology) Bhullar, Raj (Oral Biology)|
|Graduation Date: ||October 2012|
|Issue Date: ||28-Aug-2012|
|Abstract: ||Group A rotaviruses are a major cause of acute gastroenteritis in children. Almost all children are infected by the age of 5 years old. Rotavirus disease causes around 600,000 deaths per year. VP4 (P) and VP7 (G) genotypes were analyzed for prevalence and potential antigenicity, as they are known to elicit a neutralizing antibody response during infection. This study predicted the effectiveness of two recently licensed rotavirus vaccines based on Canadian surveillance.
271 out of 348 diarrhea samples from 8 paediatric hospital were successfully genotyped by PCR. Canadian rotavirus genotypes were found to be mostly G1P followed by G3P, G2P, G9P, G4P, and G9P, between 2007 and 2010.
Reassortment and motif analysis was done with a subset of rotavirus-positive samples. There were no unusual reassortment events found in Canadian strains. Variations amongst strains were commonly genotype-specific, but otherwise rare. In conclusion, rotavirus vaccine escape is presently unlikely amongst Canadian strains.|
|Appears in Collection(s):||FGS - Electronic Theses & Dissertations (Public)|
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