Effect of different carbohydrate and nucleotide derived from yeast products, including distillers dried grains with soluble (DDGS), on innate immunity using a chicken B cells model: in vitro studies

Abstract

The poultry industry has been using antibiotics to enhance bird performance through the control of microbial agents in the gastrointestinal tract; however antibiotic alternatives must be investigated. The effect of yeast-derived products such as brewer’s yeast (Y), yeast cell wall polysaccharide-rich product (YCW), wheat/corn distiller dried grains with solubles (DDGS), nucleotide-rich product (N), processed yeast/nucleotide-rich product (PY+N) and D+mannose (M) was assessed using chicken B cells (DT40 cell line) and a LPS challenge model. Relative gene expression of toll-like receptors (TLR) and cytokines was then characterized.. These results show that yeast and yeast-derived products stimulate LPS-challenged B cells to produce Th2-associated cytokines which may lead to in vivo antibody production and gram-negative bacteria clearance. Furthermore, the up-regulation of IL-10 caused by the presence of Y, DDGS, N and PY+N is an important finding as it illustrates the regulatory activity stimulated by these treatments in the presence of Escherichia coli-derived LPS.