MSpace - DSpace at UofM >
Faculty of Graduate Studies (Electronic Theses and Dissertations) >
FGS - Electronic Theses & Dissertations (Public) >

Please use this identifier to cite or link to this item: http://hdl.handle.net/1993/800

Title: An investigation and evaluation of photosynthetic and respiratory measurements as determined from pCO2 changes of incubated culture and lake water samples
Authors: Davies, John Mark
Issue Date: 1-Apr-1997
Abstract: A new method for measuring net community primary productivity and dark respiration was examined using steady state cultures and tested under field conditions. The method is based on measuring changes of pCO$\sb2$ in the headspace of a sealed incubation bottle by gas chromatography over the course of an incubation. At the end of the incubation the sample is acidified and the total DIC in the bottle is determined. Using the defined relationships between pCO$\sb2,$ DIC, and alkalinity (Park 1969) DIC is calculated for each sampling time with the assumption that alkalinity change is not significant. Nitrogen-limited, phosphorous-limited, and nitrogen and phosphorous limited cultures of Chlamydomonas reinhardtii were grown in chemostats, and subsamples were incubated using the pCO$\sb2$ method. Principal component analysis was used to evaluate expected rates of photosynthesis based on changes in particulate carbon with pCO$\sb2$ measured rates. Field measurements were conducted at the Experimental Lakes Area (ELA), Northwestern Ontario. Photosynthesis-irradiance curves were generated for all but two dates. (Abstract shortened by UMI.)
URI: http://hdl.handle.net/1993/800
Appears in Collection(s):FGS - Electronic Theses & Dissertations (Public)

Files in This Item:

File Description SizeFormat
mq23269.pdf7.37 MBAdobe PDFView/Open
View Statistics

Items in MSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

 

Valid XHTML 1.0! MSpace Software Copyright © 2002-2010  Duraspace - Feedback