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Title: Innovative approaches to assessing seed quality in Brassicas
Authors: Larkin, Jodi
Supervisor: McVetty, Peter (Plant Science)
Examining Committee: Li, Genyi (Plant Science) Belmonte, Mark (Biological Sciences)
Graduation Date: May 2012
Keywords: Brassica napus
chlorophyll concentration
erucic acid genes
near infrared reflectance spectroscopy
molecular markers
Issue Date: 2-Apr-2012
Abstract: Brassica napus is grown as an edible oil (canola) and an industrial oil (HEAR). Its fatty acid profile and chlorophyll concentration affect the quality. It is important to develop accurate and efficient methods to evaluate these traits. The first objective of this study was to improve the single nucleotide polymorphism (SNP) and sequence characterized amplified region (SCAR) molecular markers for erucic acid genotypes in the Bn-FAE1.1 and Bn-FAE1.2 genes in the A and C genomes in Brassica napus originally developed by Rahman et al. (2008). When put into practice, the error rate was unacceptably high. With the modifications that were made to the protocols, the overall accuracy remained relatively consistent indicating that further improvements are still required. The second objective was to develop a near infrared reflectance (NIR) based calibration equation for chlorophyll concentration in whole Brassica napus seeds. In this case, an equation was successfully created.
Appears in Collection(s):FGS - Electronic Theses & Dissertations (Public)

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