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Title: Comparative redox proteomics to investigate role of Nox mediated redox signaling in Fusarium graminearum pathogenesis
Authors: Joshi, Manisha
Supervisor: Rampitsch, Christof (Biochemistry and Medical Genetics)
Examining Committee: Wilkins, John (Medicine and Immunology) Gietz, Daniel (Biochemistry and Medical Genetics)
Graduation Date: October 2011
Keywords: Fusarium graminearum
Fusarium Head Blight
NADPH oxidases
Redox signaling
Issue Date: 9-Aug-2011
Abstract: Fusarium graminearum causes Fusarium Head Blight, (one of) the most destructive cereal diseases in Canada. Yield loss, quality degradation and mycotoxin production make Fusarium a multifaceted threat. Regulated production of reactive oxygen species by Nox enzymes is indispensable for fungal pathogenesis. F. graminearum Nox mutant ∆noxAB produced equivalent mycotoxin but caused reduced virulence than wild-type. We hypothesized that Nox mediated redox signaling may participate in F. graminearum pathogenicity. Two-DE and gel-free biotin affinity chromatography, followed by LC-MS/MS analysis were employed for a comparative redox-proteomics analysis between wild-type and ∆noxAB to identify proteins oxidized by Nox activity. Total 35 proteins, 10 by 2-DE and 29 by gel-free system, were identified. 34% proteins participated in fungal metabolism, 20% in electron transfer reactions and 9% were anti-oxidant proteins. The findings suggested that Nox mediated thiol-disulfide exchange in proteins provide a switch for redox-dependent regulation of metabolic and developmental processes during induction of FHB.
Appears in Collection(s):FGS - Electronic Theses & Dissertations (Public)

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