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Title: Solubility and Conformational Studies of the Intrinsically Disordered HIV-1 Tat1-72 Protein
Authors: Babiak, Taras
Supervisor: O'Neil, Joe (Chemistry)
Examining Committee: Stetefeld, Jorg (Chemistry) Mark, Brian (Microbiology)
Graduation Date: May 2011
Keywords: HIV-1
Intrinsically Disordered
Circular Dichroism
Issue Date: 20-Apr-2011
Abstract: Tat1-72, is an intrinsically disordered protein at pH 4.1 as previously indicated by NMR chemical shifts and coupling constants, and confirmed by 15N-relaxation parameters. The presence of SDS elicits a conformational change to α-helicity in Tat1-72. In the presence of the non-ionic DDM detergent and zinc, Tat was found to be soluble at pH 4 when bound to TAR RNA; TAR binding also elicits a conformational shift to α-helicity in Tat1-72. The β-sheet content of Tat1-72 is increased in the presence of NaCl. In similar conditions, Tat1-72 aggregates stained with Congo Red displayed a yellow-green birefringence and a red-shift in the Congo Red absorbance that is typical of β-amyloid fibril. The web-based algorithm “WALTZ” identifies the majority of the Tat1-72 hydrophobic core region as amyloidogenic. The helical propensity of Tat1-72 in TFE was determined by two-dimensional NMR spectroscopy.
Appears in Collection(s):FGS - Electronic Theses & Dissertations (Public)

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