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Title: Identification and characterization of new cellular interacting proteins of HIV-1 integrase
Authors: Parvez, Md. Kamal Uddin
Supervisor: Yao, Xiaojian (Medical Microbiology)
Examining Committee: Fowke, Keith (Medical Microbiology) Peng, Zhikang (Immunology) Cheng, keding (Human Anatomy & Cell Science)
Graduation Date: May 2011
Keywords: HIV-1
Issue Date: 12-Apr-2011
Abstract: HIV-1 integrase (IN) enzyme employs several viral and cellular proteins for nuclear translocation and crucial integration of viral cDNA. Successful identification of new viral/cellular interactions may shed light for better understanding of HIV-1 replication. 293T cells were transiently transfected with pYEF-1-TAP-IN and cell lysate were subjected to Tandem Affinity Purification system to pull down putative IN-interacting cellular partners. A number of distinct bands from the Coomassie-stained gel were excised followed by in-gel digestion and mass spectrometry. Putative cellular partners of HIV-1 IN were heat shock protein 60 (HSP60), β-tubulin, γ-actin, ATP synthase alpha subunit and histone H1.2 were identified by mass spectrometry. Additionally, SF3A3 (splicing factor 3A3), another previously reported factor, was successfully co-immunoprecipitated with IN. The C-terminal portion of IN was found to be the region of interaction with SF3A3. Overall, this study has provided better understanding of IN dynamics enriching existing knowledge of HIV-1 IN biology.
Appears in Collection(s):FGS - Electronic Theses & Dissertations (Public)

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