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|Title: ||NeuroImmune modulation of multiple sclerosis via the dorsal root ganglia|
|Authors: ||Melanson, Maria|
|Supervisor: ||Namaka, Mike (Pharmacy)|
|Examining Committee: ||Kreillaars, Dean (Medical Rehabilitation) Shay, Barbara (Medical Rehabilitation)|
|Graduation Date: ||May 2011|
|Keywords: ||TNS alpha, tumor necorsis factor, dorsal root ganglia, EAE|
|Issue Date: ||11-Apr-2011|
|Citation: ||Melanson, Maria (2008). Axotomy-induced up-regulation of tumor necrosis factor-alpha in the dorsal root ganglia. Neurol Res. Jul;30(6):623-31, Epub 2008 May 16.|
|Abstract: ||Background: Multiple sclerosis (MS) is a chronic, neurological disease characterized by targeted destruction on central nervous system (CNS) myelin. The autoimmune theory is the most widely accepted explanation of disease pathology. Circulating Th-1 cells become activated by exposure to CNS-specific antigens such as myelin basic protein. The activated Th-1 cells secrete inflammatory cytokines, which are pivotal for inflammatory responses. We hypothesize that enhanced production of inflammatory cytokines triggers cellular events within the dorsal root ganglia (DRG) and/or spinal cord, facilitating the development of neuropathic pain (NPP) in MS. NPP, the second worst disease-induced symptom suffered by patients with MS, is normally regulated by DRG and/or spinal cord.
Objective: To determine gene and protein expression levels of tumor necrosis factor-alpha (TNF ) within DRG and/or spinal cord in an animal model of MS.
Methods: Experimental autoimmune encephalomyelitis (EAE) was induced in adolescent female Lewis rats. Animals were sacrificed every 3 days post-disease induction. DRG and spinal cords were harvested for protein and gene expression analysis.
Results: We show significant increases in TNF expression in the DRG and of EAE animals at peak disease stage, as assessed by clinical symptoms.
Conclusion: Antigen-induced production of inflammatory cytokines such as TNF within the DRG identifies a potential noel mechanism for MS-induced NPP.|
|Appears in Collection(s):||FGS - Electronic Theses & Dissertations (Public)|
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