A microflow cytometer with simultaneous dielectrophoretic actuation for the optical assay and capacitive cytometry of individual fluid suspended bioparticles
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Date
2009-09-14T21:24:54Z
Authors
Romanuik, Sean
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Abstract
Fluid suspended biological particles (bioparticles) flowing through a non-uniform electric field are actuated by the induced dielectrophoretic (DEP) force, known to be dependent upon the bioparticles’ dielectric phenotypes. In this work: a 10-1000 kHz DEP actuation potential applied to a co-planar microelectrode array (MEA) induces a DEP force, altering passing bioparticle trajectories as monitored using: (1) an optical assay, in which the lateral bioparticle velocities are estimated from digital video; and (2) a capacitive cytometer, in which a 1.478 GHz capacitance sensor measures the MEA capacitance perturbations induced by passing bioparticles, which is sensitive to the bioparticles’ elevations. The experimentally observed and simulated lateral velocity profiles of actuated polystyrene microspheres (PSS) and viable and heat shocked Saccharomyces cerevisiae cells verify that the bioparticles’ dielectric phenotypes can be inferred from the resultant trajectories due to the balance between the DEP force and the viscous fluid drag force.
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Keywords
Microflow, Microfluidic, Cytometer, Cytometry, Dielectrophoretic, Dielectrophoresis, Interferometer, Interferometric, Capacitive Sensor, Capacitance Sensing, Capacitive Detector, Capacitance Detection, Polystyrene, Optical Assay, Electrokinetic, Actuation, Yeast, Saccharomyces cerevisiae, Single-cell Diagnostic, Single-cell Diagnosis, Dielectric Modeling, COMSOL, Tracker, Trajectory, Velocity Profile, Capacitive Signature, Capacitance Signature, Microelectrode
Citation
Ferrier, G.A., A.N. Hladio, D.J. Thomson, G.E. Bridges, M. Hedayatipoor, S. Olson, and M.R. Freeman (2007). Microfluidic electromanipulation with capacitive detection for cell diagnositc applications, Proc. NSTI 2:214-217.
Ferrier, G.A., S.F. Romanuik, D.J. Thomson, G.E. Bridges, and M.R. Freeman (2008). Capacitance signatures for rapid detection of the polarity of the dielectrophoretic force on single yeast cells, Proc. NSTI 2:589-592.
Ferrier, G.A., A.N. Hladio, D.J. Thomson, G.E. Bridges, M. Hedayatipoor, S. Olson, and M.R. Freeman (2008). Microfluidic electromanipulation with capacitive detection for the mechanical analysis of cells, Biomicrofluidics 2(4-044102):1-13.
Ferrier, G.A., S.F. Romanuik, D.J. Thomson, G.E. Bridges, and M.R. Freeman (Submitted 2009). A microwave interferometric system for simultaneous actuation and detection of single biological cells, Lab on a Chip.
Ferrier, G.A., S.F. Romanuik, D.J. Thomson, G.E. Bridges, and M.R. Freeman (Submitted 2009). Rapid classification of biological cells based on dielectrophoretic actuation with simultaneous capacitive detection, Proc. micro-TAS.
Nikolic-Jaric, M., S.F. Romanuik, G.A. Ferrier, G.E. Bridges, M. Butler, K. Sunley, D.J. Thomson, and M.R. Freeman (2009). Microwave frequency sensor for detection of biological cells in microfluidic channels, Biomicrofluidics 3(3-034103):1-15.
Romanuik, S.F., G.A. Ferrier, D.J. Thomson, G.E. Bridges, S. Olson, and M.R. Freeman (2008). All-electronic detection and actuation of single biological cells for lab-on-a-chip applications, Proc. IEEE Sensors 1:634-637.
Romanuik, S.F., G.A. Ferrier, M.N. Jaric, D.J. Thomson, G.E. Bridges, and M.R. Freeman (Submitted 2009). Dielectrophoretic actuation and simultaneous detection of individual bioparticles, Advances in Biomedical Sensing, Measurements, Instrumentation, and Systems, Eds. A. Lay-Ekuakille and S.C. Mukhopadhyay, Springer – Verlag.
Ferrier, G.A., S.F. Romanuik, D.J. Thomson, G.E. Bridges, and M.R. Freeman (2008). Capacitance signatures for rapid detection of the polarity of the dielectrophoretic force on single yeast cells, Proc. NSTI 2:589-592.
Ferrier, G.A., A.N. Hladio, D.J. Thomson, G.E. Bridges, M. Hedayatipoor, S. Olson, and M.R. Freeman (2008). Microfluidic electromanipulation with capacitive detection for the mechanical analysis of cells, Biomicrofluidics 2(4-044102):1-13.
Ferrier, G.A., S.F. Romanuik, D.J. Thomson, G.E. Bridges, and M.R. Freeman (Submitted 2009). A microwave interferometric system for simultaneous actuation and detection of single biological cells, Lab on a Chip.
Ferrier, G.A., S.F. Romanuik, D.J. Thomson, G.E. Bridges, and M.R. Freeman (Submitted 2009). Rapid classification of biological cells based on dielectrophoretic actuation with simultaneous capacitive detection, Proc. micro-TAS.
Nikolic-Jaric, M., S.F. Romanuik, G.A. Ferrier, G.E. Bridges, M. Butler, K. Sunley, D.J. Thomson, and M.R. Freeman (2009). Microwave frequency sensor for detection of biological cells in microfluidic channels, Biomicrofluidics 3(3-034103):1-15.
Romanuik, S.F., G.A. Ferrier, D.J. Thomson, G.E. Bridges, S. Olson, and M.R. Freeman (2008). All-electronic detection and actuation of single biological cells for lab-on-a-chip applications, Proc. IEEE Sensors 1:634-637.
Romanuik, S.F., G.A. Ferrier, M.N. Jaric, D.J. Thomson, G.E. Bridges, and M.R. Freeman (Submitted 2009). Dielectrophoretic actuation and simultaneous detection of individual bioparticles, Advances in Biomedical Sensing, Measurements, Instrumentation, and Systems, Eds. A. Lay-Ekuakille and S.C. Mukhopadhyay, Springer – Verlag.