MSpace - DSpace at UofM >
University of Manitoba Researchers (Research Publications) >
Research Publications (UofM Student, Faculty and Staff only access) >

Please use this identifier to cite or link to this item: http://hdl.handle.net/1993/2938

Title: Sequence and chromosomal assignment of a human novel cDNA: similarity to gamma-aminobutyric acid transporter
Authors: Gong, YW
Zhang, MN
Cui, L
Minuk, GY
Keywords: sequence
chromosome
GABA
GABA transporter
HEPATIC REGENERATIVE ACTIVITY
GABA LEVELS
RAT-BRAIN
ENCEPHALOPATHY
CLONING
SYSTEM
EXPRESSION
PLASMA
DISEASE
BINDING
Issue Date: 31-Dec-2001
Citation: 0008-4212; CAN J PHYSIOL PHARMACOL, DEC 2001, vol. 79, no. 12, p.977 to 984.
Abstract: Gamma-aminobutyric acid (GABA) is the predominant inhibitory neurotransmitter in the mammalian brain. Although initially thought to be confined to the central nervous system, GABAergic activity has also been described in other tissues throughout the body. In the present study, we report the cloning and localization of human GABA transporter cDNA and document its expression in various human tissues. A human liver cDNA library was initially screened by a P-32-labeled murine brain GABA transporter 3 (GAT-3) cDNA probe, and full-length cDNA was cloned by employing Marathon-Ready(TM) human kidney cDNA. The human GABA transporter cDNA encoded a 569 amino acid hydrophobic protein with 12 transmembrane domains (TMs). Search of published sequences revealed high homology with rat GAT-2, murine GAT-3 cDNA, human solute carrier family 6 member 13 (SLC6A13), and a human peripheral betaine/GABA transporter. Northern blot analyses demonstrated that the human GABA transporter is expressed strongly in the kidney and to a lesser extent in the liver and brain. The sequence was well matched with human chromosome 12p13.3, suggesting the human GABA transporter contains 14 exons. The above findings confirm the existence of and further characterize a specific GABA transporter in human tissues.
URI: http://hdl.handle.net/1993/2938
Appears in Collection(s):Research Publications (UofM Student, Faculty and Staff only access)

Files in This Item:

File Description SizeFormat
Gong_Sequence_and_chromosomal.pdf1.88 MBAdobe PDFView/Open
View Statistics

Items in MSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

 

Valid XHTML 1.0! MSpace Software Copyright © 2002-2010  Duraspace - Feedback