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Please use this identifier to cite or link to this item: http://hdl.handle.net/1993/2928

Title: Multiple environmental factors regulate the expression of the carbohydrate-selective OprB porin of Pseudomonas aeruginosa
Authors: Adewoye, LO
Worobec, EA
Keywords: Pseudomonas aeruginosa
OprB porin
glucose transport
regulation
ESCHERICHIA-COLI K-12
OUTER-MEMBRANE PROTEIN
GENE-EXPRESSION
TRANSPORT-SYSTEM
CHANNEL SIZE
SIGNAL TRANSDUCTION
OSMOTIC REGULATION
MOLECULAR ANALYSIS
CYSTIC-FIBROSIS
PH
Issue Date: 31-Dec-1999
Citation: 0008-4166; CAN J MICROBIOL, DEC 1999, vol. 45, no. 12, p.1033 to 1042.
Abstract: In response to low extracellular glucose concentration, Pseudomonas aeruginosa induces the expression of the outer membrane carbohydrate-selective OprB porin. The promoter region of the oprB gene was cloned into a lacZ transcriptional fusion vector, and the construct was mobilized into P. aeruginosa OprB-deficient strain, WW100, to evaluate additional environmental factors that influence OprB porin gene expression. Growth temperature, pH of the growth medium, salicylate concentration, and carbohydrate source were found to differentially influence porin expression. This expression pattern was compared to those of whole-cell [C-14]glucose uptake under conditions of high osmolarity, ionicity, variable pH, growth temperatures, and carbohydrate source. These studies revealed that the high-affinity glucose transport genes are down-regulated by salicylic acid, differentially regulated by pH and temperature, and are specifically responsive to exogenous glucose induction.
URI: http://hdl.handle.net/1993/2928
DOI: http://dx.doi.org/10.1139/w99-110
Appears in Collection(s):Research Publications (UofM Student, Faculty and Staff only access)

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