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Please use this identifier to cite or link to this item: http://hdl.handle.net/1993/2927

Title: Cytotoxic effects of pyocin S2 produced by Pseudomonas aeruginosa on the growth of three human cell lines
Authors: Abdi-Ali, A
Worobec, EA
Deezagi, A
Malekzadeh, F
Keywords: Pseudomonas aeruginosa
pyocin
cytotoxicity
MTT assay
TRANSFERRIN RECEPTORS
COLORIMETRIC ASSAY
PURIFICATION
BACTERIOCIN
COLICIN
NEOPLASIA
PROTEINS
GENES
VITRO
PAO1
Issue Date: 31-May-2004
Citation: 0008-4166; CAN J MICROBIOL, MAY 2004, vol. 50, no. 5, p.375 to 381.
Abstract: Pyocin typing of 82 Pseudomonas aeruginosa strains, collected from different Iranian clinical sources, revealed that one isolate, P. aeruginosa 42A, produced pyocin S2, a protease-sensitive bacteriocin. Pyocin S2 production was induced by mitomycin C (2 mug/mL) in the pyocin S2 producer P. aeruginosa 42A. Pyocin S2 was purified using ion exchange chromatography with CM-Sepharose CL-6B and sodium phosphate buffer (pH 8) from an 80% ammonium sulfate precipitate of whole-cell lysates. Pyocin activity of the fractions was detected using the Govan spot testing method. The purity of the active fraction was confirmed by SDS-PAGE, where a single band with a molecular mass of 74 kDa was detected. Cytotoxic effects of purified pyocin S2 and partially purified pyocin from P. aeruginosa 42A on the human tumor cell lines HepG2 and Im9 and the normal human cell line HFFF (Human Foetal Foreskin Fibroblast) were studied by the MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay. The results demonstrated that partially purified pyocin and pyocin S2 exhibited substantial inhibitory effects on the growth of the tumor cell lines HepG2 and Im9, while no inhibitory effects were observed on the normal cell line HFFF. Pure lipopolysaccharide was used as a control and was found to have no inhibitory effect on any of the cell lines tested.
URI: http://hdl.handle.net/1993/2927
DOI: http://dx.doi.org/10.1139/W04-019
Appears in Collection(s):Research Publications (UofM Student, Faculty and Staff only access)

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