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Please use this identifier to cite or link to this item: http://hdl.handle.net/1993/2912

Title: Effects of mitogen-activated protein kinases on nuclear protein import
Authors: Faustino, RS
Rousseau, DC
Landry, MN
Kostenuk, AL
Pierce, GN
Keywords: mitogen activated protein kinase
nuclear protein import
cell proliferation
nucleus
cell growth
PORE COMPLEX
MAP KINASE
TRANSPORT
PHOSPHORYLATION
INHIBITION
PATHWAY
RANGAP1
RAN
NUCLEOPORINS
INVOLVEMENT
Issue Date: 30-Apr-2006
Citation: 0008-4212; CAN J PHYSIOL PHARMACOL, MAR-APR 2006, vol. 84, no. 39145, p.469 to 475.
Abstract: ERK-2 MAP kinase activation induces inhibitory effects on nuclear protein import in vascular smooth muscle cells. The mechanism and characteristics of this effect of ERK-2 were investigated. An Unusual dose-dependent effect of ERK-2 on nuclear protein import was identified. At higher concentrations (1 mu g/mL) of ERK-2, nuclear protein import was stimulated. whereas lower concentrations (0.04 mu g/mL) inhibited import. Intermediate concentrations exerted intermediate effects. The stimulatory and inhibitory, effects at the 2 different ERK-2 concentrations were observed in both conventional. permeabilized cell assays of nuclear protein import and With in situ microinjection of smooth muscle cells. The biphasic effects of ERK-2 oil import were also found for the other 2 members of the MAPK family. p38 and JNK. Ran-GAP was identified by structural analysis as a candidate target protein responsible for mediating the effects of ERK-2. After pretreatment with high concentrations of ERK-2. RanGAP activity was significantly increased by similar to 50%. In contrast. low concentrations of ERK-2 significantly attenuated RanGAP activity. These results demonstrate that all 3 members of the MAPK family can alter nuclear protein import in opposite directions depending upon the concentration of ERK-2 Used. RanGAP represents the MAP kinase target whereby nuclear transport can be stimulated or inhibited.
URI: http://hdl.handle.net/1993/2912
DOI: http://dx.doi.org/10.1139/y05-131
Appears in Collection(s):Research Publications (UofM Student, Faculty and Staff only access)

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