MSpace - DSpace at UofM >
University of Manitoba Researchers (Research Publications) >
Research Publications (UofM Student, Faculty and Staff only access) >

Please use this identifier to cite or link to this item: http://hdl.handle.net/1993/2779

Title: Unusual compact rDNA gene arrangements within some members of the Ascomycota: evidence for molecular co-evolution between ITS1 and ITS2
Authors: Hausner, G
Wang, X
Keywords: rDNA
ITS1 and ITS2
Ascomycota
co-evolution
INTERNAL TRANSCRIBED SPACER
PRE-RIBOSOMAL-RNA
SCHIZOSACCHAROMYCES-POMBE
SPHAERONAEMELLA-FIMICOLA
SACCHAROMYCES-CEREVISIAE
CONCERTED EVOLUTION
SECONDARY STRUCTURE
FUNCTIONAL-ANALYSIS
DNA
SEQUENCE
Issue Date: 6-Aug-2005
Citation: 0831-2796; GENOME, AUG 2005, vol. 48, no. 4, p.648 to 660
Abstract: The internal transcribed spacers of the ribosomal DNA tandem repeat were examined in members of the ascomycetous genus Sphaeronaemella. Species of Sphaeronaemella and its mitotic counterpart Gabarnaudia, have a compact rDNA gene arrangement due to unusually short internal transcribed spacer (ITS) regions. Examination of these regions from phylogenetically related taxa, Cornuvesica, Gondwanamyces, and Ceratocystis, showed that their ITS1 and ITS2 regions could be folded into central hairpin-like structures with the size reduction in species of Sphaeronaemella being due to length reduction of the main-hairpin and the loss of smaller hairpin-like structures that emanate from the main hairpin. A databank compilation, combined with newly obtained sequences, provided an ITS data set that includes sequences of 600 species belonging to the Ascomycota. Correlation analysis revealed that the sizes of ITS1 and ITS2 show a strong positive correlation, suggesting that the 2 rDNA regions have co-evolved. This supports biochemical evidence indicating that the ITS1 and ITS2 segments interact to facilitate the maturation of the rRNA precursor.
URI: http://hdl.handle.net/1993/2779
DOI: http://dx.doi.org/10.1139/g05-037
Appears in Collection(s):Research Publications (UofM Student, Faculty and Staff only access)

Files in This Item:

File Description SizeFormat
Hausner_Unusual_compace_rDNA.pdf1.5 MBAdobe PDFView/Open
View Statistics

Items in MSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

 

Valid XHTML 1.0! MSpace Software Copyright © 2002-2010  Duraspace - Feedback