MSpace - DSpace at UofM >
Faculty of Graduate Studies (Electronic Theses and Dissertations) >
FGS - Electronic Theses & Dissertations (Public) >

Please use this identifier to cite or link to this item: http://hdl.handle.net/1993/2055

Title: The generation and characterization of avian reovirus temperature sensitive mutants
Authors: Patrick, Megan Kathleen
Issue Date: 1-May-2001
Abstract: Currently, little is known about the genetic and biological functions of avian reovirus (ARV), an atypical member of the family 'Reoviridae ' and the prototype of all non-enveloped syncytia-inducing viruses. In this study, we created temperature sensitive ('ts') mutants by chemical mutagenesis of ARV strain 138. We developed a novel efficiency of lysis (EOL) technique to screen for temperature sensitivity and used the classical efficiency of plating (EOP) assay to identify 17 ARV 'ts ' mutants. Pairwise mixed infection of these mutants and evaluation of recombinant progeny 'ts' status led to their organization into 7 recombination groups. This indicates that these new groups of ARV mutants represent the majority of the ARV genome. To phenotypically characterize each mutant and localize the effects of the 'ts' lesion within the viral lifecycle, the ability to synthesize double stranded RNA (dsRNA) at permissive (33.5C) and non-permissive temperature (39.5C) was analyzed. (Abstract shortened by UMI.)
URI: http://hdl.handle.net/1993/2055
Appears in Collection(s):FGS - Electronic Theses & Dissertations (Public)

Files in This Item:

File Description SizeFormat
MQ57570.pdf6.15 MBAdobe PDFView/Open
View Statistics

Items in MSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

 

Valid XHTML 1.0! MSpace Software Copyright © 2002-2010  Duraspace - Feedback